ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A bioelectrode consisting of two parallel noble metal nets and a thin layer of gel-entrapped urease between them permits to determine urea conductimetrically with a response time 3-5 min and reproducibility 5-6% above 60 micromolar concentration of urea. Differential measurement eliminates the interference due to conductive components of the real sample. Evidence has been obtained for extended application of the method by using immobilized microbial cells or tissue slice as biocatalyst in place of the immobilized purified enzyme. It is also suitable for simple kinetical assaying arginase activity in the tissue extracts.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370090416
