ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The configuration and applicability of a system for on-line determinations of media components by high performance liquid chromatography (HPLC) is described, assembled only from commercial available stand-alone devices. The fermentation broth is circulated in a bypass through a magnetically stirred filtration unit. The clear filtrate is injected at preset intervals by an electrically actuated valve into the HPLC analysis system. Excess filtrate is circulated back to the fermenter to minimize liquid loss. The system offers aseptic operation, an excellent long-term stability and a relatively short response time. A wide range of media components is detectable by appropriate choice of analytical HPLC columns and detectors. The system was tested for the analysis of carbohydrates in cultivations of Saccharomyces cerevisiae, Streptomyces olivaceus and Arthrobacter sp.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370100502
