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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 208 (1984), S. 233-242 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Insulin, glucagon, somatostain, and pancreatic polypeptide (PP) were localized in the pancreas of the common garter snake, Thamnophis sirtalis, by light and transmission electeron microscopic (TEM) immunocytochemistry. Colloidal gold-protein A was used for TEM localization and the peroxidase containing A cells and the insulin-positive B cells were the most numerous cell types. The somatostatin-containing D cells made up about 15% of the endocrine cells. PP-positive F cells were a minor cell type. The only topographic arrangement of the cells within the endocrine-rich areas that was apparent was the peripheral localization of the D and F cells. Cells of a specific cell type were sometimes grouped together. At the elctron microscopic (EM) level, the gold particles (indicating the pesence of hormone) were localized nearly exclusively over the secretory grnules of the ractive cells. The α-granules were the largest found and were predominantly electron dense with a moderately electron-dense periphery. PP-containing granules were the smallest. The somatostatin-reactive δ-granules were round and moderately electron opaque. The β-granules were heterogeneous in appearance. The morphognomy of the secretory granules of the major endocrine cell types is qualitatively similar to that of mammals. Wheter or not the quantitative and/or associative differences contribute to the marked metabolic differences between reptiles and mammals, remains to be determined.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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