ISSN:
0886-1544
Keywords:
microtubules
;
microfilaments
;
filopodia
;
cell spreading
;
coelomocytes
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Sea urchin coelomocytes were used as a model system to investigate the distribution and role of microtubules and microfilaments in cell spreading and filopodial formation. By using immunoblot characterized antisera to tubulin and actin coupled with immunofluorescence techniques, cellular protrusions were seen to contain actin filaments but no microtubules. Cells depleted of MT's by cold and colcemid treatments could attach, spread, and transform to the filopodial morphology normally.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970040405
