ISSN:
1432-0428
Keywords:
Insulin receptor
;
glucocorticoid responsive element
;
gel mobility shift analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The interaction of nuclear protein extracted from rat liver and 5′-flanking DNA of the human insulin receptor gene was investigated with the aid of gel mobility shift analysis. When 5′-flanking DNA (-1255/-1206 or -385/-345 base pairs) was incubated with nuclear protein, two or three 32P-DNA species (protein binding DNA fragment(s) and free DNA fragment) were detected. These bands did not disappear in spite of increasing amounts of synthetic poly(dI-dC), showing that nuclear protein binds specifically to 5′-flanking DNA of the insulin receptor gene. Increasing amounts of long terminal repeat of mouse mammary tumour virus resulted in a reciprocal decrease in nuclear protein binding to 5′-flanking DNA of insulin receptor gene. These results suggest that 5′-flanking DNA of insulin receptor gene binds to the same nuclear protein to which long terminal repeat of mouse mammary tumour binds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00297453
