ISSN:
1432-0428
Keywords:
Key words Diabetic nephropathy, glomerular mesangial cells, protein kinase C, isoenzymes, high glucose.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The activities and expression of protein kinase C isoenzymes were examined in glomerular mesangial cells cultured under high glucose conditions. Exposure of cells to high glucose concentrations (27.8 mmol/l) for more than 3 days resulted in a significant elevation of protein kinase C activities in the membrane fraction. Of the protein kinase C isoenzymes, the levels of protein kinase C α significantly increased in the membrane fraction after 3 days of exposure to glucose, and protein kinase C ζ increased after 5 days of exposure. Levels of protein kinase C δ and ɛ remained unchanged and protein kinase C β and γ were not detected. These results indicate that protein kinase C α and ζ are translocated under high glucose conditions possibly through different mechanisms. [Diabetologia (1994) 37: 838–841]
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00404342