ISSN:
1432-0428
Keywords:
Solid phase radioimmunoassay
;
immunoradiometric assay
;
labelled antibody
;
two site assay
;
antigen localisation
;
antibody estimation
;
cell membranes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Solid phase biochemical systems have many technical advantages. The lecture reviews a number of ways in which solid phase immunological methods may be used in diabetes research. Solid phase antibodies are very convenient for use in radioimmunoassay. The immunoradiometric assay involves the isolation of antibodies onto solid phase antigen, the iodination of the antibodies whilst combined to the solid phase antigen, the elution of the iodinated antibodies and finally their use as a reagent for the measurement of antigen. The two site assay utilises solid phase antibodies to extract and concentrate antigen from solutions such as plasma. The extracted antigen may then be assayed whilst combined with solid phase antigen if a second site on the antigen is available for reaction with I125 — labelled antibody. High specific activity, purified labelled antibodies may also be used for antigen localization, antibody estimation and labelling cell membranes. Cell membrane purification using solid phase antibodies is considered as a further development of this methodology.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01225565
