ISSN:
1432-1106
Keywords:
Dentate gyrus culture
;
Non-granule cells
;
High-voltage-activated Ca2+ current
;
Delayed rectifier K+ current
;
Ca2+-dependent K+ current
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Electrophysiological properties of neurofilament-positive neurones in dissociated cell cultures were prepared at postnatal days 4–5 from rat dentate gyrus and studied using the whole-cell patch-clamp technique. These cells expressed a fast-inactivating, 0.5 μM tetrodotoxin-sensitive Na+ current; a high-voltage-activated (HVA) Ca2+ current, which was 30 μM Cd2+- and partially 2 μM nicardipine-sensitive; and an inward rectifier current, which was sensitive to extracellularly applied 1 mM Cs+. The outward current pattern was composed of a delayed rectifier-like outward current sensitive to 20 mM tetraethylammonium (TEA) and a fast-inactivating, Ca2+-dependent outward current. This transient Ca2+-dependent K+ outward current was identified by a subtraction procedure. K+ currents recorded under conditions of blocked Ca2+ currents (after rundown of the HVA Ca2+ current or blocked by extracellularly applied Cd2+) were subtracted from control currents. By comparison with the current pattern of identified dentate granule cells, it is concluded that the investigated cell type originated from interneurones or projection neurones of the dentate hilus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00230419
