ISSN:
1432-0983
Keywords:
Orotidine-5′-phosphate decarboxylase
;
Cephalosporium acremonium
;
Recombinant DNA
;
Evolution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5′-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00312613
