ISSN:
1432-0983
Keywords:
Key words Basidiomycete
;
Transformation
;
White-rot fungi
;
Carboxin
;
Succinate:ubiquinone oxidoreductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050521
