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Digitale Medien

Callus formation from protoplasts of culturedSpinacia oleracea cells (1985)

Nakagawa, H. ; Tanaka, H. ; Oba, T. ; [weitere]

Springer

Plant cell reports 4 (1985), S. 148-150

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Details

ISSN: 1432-203X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Cell suspensions were initiated from plumule derived calli ofSpinacia oleracea. Some of these cell lines could be maintained in culture for at least three years without a reduced growth rate. A high yield of protoplasts was obtained from the cell suspensions. When protoplasts were cultured in Murashige and Skoog medium with naphthaleneacetic acid and 6-benzyladenine, cell wall formation was observed after three days. The cultured protoplasts produced numerous cell-clusters within two weeks. However only protoplasts isolated from suspensions which were in a rapidly dividing phase were able to divide with a high frequency and give rise to callus colonies.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00571303

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