ISSN:
1432-1831
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A technique using antibody-coated tubes allows the radioimmunological determination of tetanus antibodies in a simple and rapid way. The procedure is based on the competition for125I-tetanus toxin between the insolubilized antibodies and the antibodies present in the unknown sample. When antibodies and125I labeled tetanus toxin are incubated in the antibody-coated polyethylene vessel the detection limit is about 0.016 IU/ml. When tetanus toxin is preincubated together with its antibody in uncoated vessels before the mixture is transferred to antibody-coated vessels, the sensitivity can be raised about tenfold. The method is especially useful for screening multiple samples.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02124342
