ISSN:
1432-1831
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The hemagglutinin (HA) and neuraminidase (NA) of influenza viruses, as well as the fusion protein (F) and hemagglutinin-neuraminidase (HN) of paramyxoviruses, have been separated in native form using a two-step procedure. The glycoproteins are efficiently extracted from virions using the non-ionic detergent octyl-Β-D-glucoside and are then applied to a column of agarose beads coupled with tyrosine-sulfanilic acid. PureHA andF are obtained in good yield in the flow-through from this column.NA andHN bind strongly and can be eluted, albeit somewhat contaminated withHA orF, by raising the pH of the column buffer. The separated non-denaturated fractions can be used for structural, functional, and antigenic studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02121808