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Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD) (1986)

Ballance, D. J. ; Turner, G.

Springer

Molecular genetics and genomics 202 (1986), S. 271-275

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ISSN: 1617-4623

Keywords: Aspergillus ; Isocitrate lyase ; Cloning ; Recombinant DNA

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation of A. nidulans. Plasmids rescued in Escherichia coli were able to transform five different A. nidulans acuD mutants. Transformation using plasmids containing the cloned fragment resulted in integration at the acuD locus in six of nine transformants.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00331649

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