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  • 1
    Electronic Resource
    Electronic Resource
    In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 546-549 
    Details
    ISSN: 1617-4623
    Keywords: Transposition ; Genomic libraries ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in-vivo into λ phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used succesfully to clone several E. coli genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338096
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