ISSN:
1617-4623
Keywords:
Recombinant DNA
;
Vaccine development
;
Carrier protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Hypervariable regions (HRs) of the major subunit of F11 fimbriae were exploited for insertion of foreign epitopes. Two insertion vectors were created that contain a unique cloning site in HR1 or HR4 respectively. Several oligonucleotides, coding for antigenic determinants derived from different pathogens, were cloned in both insertion vectors. Hybrid fimbrial subunits were generally shown to be assembled in fimbriae when the length of the inserted peptide did not exceed 14 amino acids. The inserted peptides appeared to be exposed in the fimbrial filament. One hybrid fimbrial protein induced detectable levels of antibodies against the inserted epitope if injected into mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00633832
