ISSN:
1617-4623
Keywords:
Recombinant DNA
;
Shotgun cloning
;
Filamentous fungi
;
Promoter sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00538710