ISSN:
1432-0827
Keywords:
Mesenchymol cell proliferation
;
Chondrogenesis
;
Osteogenesis
;
Proteoglycans
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Summary The influence of retinoic acid on matrix-induced endochondral bone differentiation was determined. Retinoic acid was administered during discrete stages of endochondral bone formation, specifically, mesenchymal cell proliferation, chondrogenesis, bone formation, and mineralization. In retinoic acid-treated rats examined on day 3 following matrix implantation, biochemical markers for mesenchymal cell proliferation were about 50% of the controls. Chondrogenesis on day 7, assessed by35SO4 incorporation into proteoglycans, was 27% of the control. In addition, dissociative extraction of proteoglycans with 4.0 M guanidine-HCl and chromatography on Sepharose CL-2B revealed the synthesis of a smaller molecular weight proteoglycan when compared to controls which exhibited the cartilage-specific type. Osteogenesis and bone mineralization were monitored by alkaline phosphatase activity and45Ca incorporation. On day 11 alkaline phosphatase activity was decreased by 40% and45Ca incorporation was 48% of the control. These results revealed the multiple foci of the actions of excess vitamin A.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02405115
