ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract An enzyme has been isolated fromPseudomonas marginalis (a pathogen responsible for the spoilage of fruits and vegetables in storage) that degrades the exopolysaccharide (mw〉2×106 Da) produced by this organism. The mechanism of degradation has been determined to be a depolymerization that results from glucosidic cleavage. The product of depolymerization is a polysaccharide of ca. 2.5×105 Da. The enzyme has a molecular weight of approximately 28 kDa, a pH optimum of 6.5, pI=9.1, and an apparent Km of 1.95×10−7±3.5×10−8 M. Native and enzyme-treated marginalan supported no or very limited growth of the bacterium respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01575921