ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Lactose-specific enzyme IIA of the phosphoenol:pyruvate-dependent sugar phosphotransferase system from Lactococcus lactis has been crystallized in phosphate buffer. The crystals belong to space group P41212 or its enantiomorph P43212 with unit-cell axes a = b = 90.9 and c = 82.4 Å. The packing parameter (Matthews parameter) Vm of 2.48 Å3 Da−1 is consistent with one trimer per asymmetric unit and non-crystallographic threefold symmetry has been confirmed by calculating a selfrotation function. The crystals diffract X-rays to at least 2.3 Å resolution, are stable in an X-ray beam and are therefore appropriate for structure determination. Native data to 2.3 Å resolution have been collected using a MAR image-plate system at a synchrotron source. One isomorphous heavy-atom derivative has been identified and the presence of an isomorphous signal in the data has been confirmed by Patterson methods.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444996005926
