ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Biomimetic nanoapatite coatings was developed by functionally modified methods with acombination of topographic, chemical and biomimetic treatments on the surface of titanium (Ti)substrate. The biological behavior and bioactivity of functionally modified SLA implants withchemical and biomimetic treatments (SCB-treated Ti) using body like solution were investigated tocompare with untreated Ti and SLA Ti plates as controls. The cell attachment, proliferation,alkaline phosphotatse (AKP) activity, cell morphology and differentiation were evaluated by usingMTT, RT-PCR, scanning electron microscopy (SEM) and confocal laser-scanning microscope(CLSM) analysis system. The results showed that the cell adhesion and proliferation was enhancedon functionalized titanium surface with nano-scale apatite compared to the controls. SEMmicrographs also revealed that the osteoblast-like cells spreadly grew along the surface. Cellmorphology and differentiation could be further observed distinctly by CLSM graphs. Moreover,mRNA expression of alkaline phosphotatse in nucleus on the SCB-treated Ti increased obviouslyon the third day compared with the controls. The in vitro results demonstrated the remarkableimprovement on cell adhesion and proliferation of the biomimetic nanoapatite on SCB-treated Ti,which could be used for orthopaedic/dental implants
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.385.pdf
