ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Hydroxyapatite (HA) coatings were introduced onto Poly L-lactic Acid (PLLA) polymerin a controlled manner by immobilized urease method with a shortened precipitation time.Osteoblastic-like cellular responses to the composite were examined in terms of cell proliferation,differentiation and cell morphology, as well as the expression of bone-associated genes. The cellsexhibited higher cellular proliferation at 2 and 4 days on the HA/PLLA composite compared toPLLA scaffold, while no significant difference was observed later at 6 days. The alkalinephosphatase (ALP) activity by cells at 7 days was statistically higher on HA/PLLA scaffold than onPLLA. Moreover, the gene expression of ALP and osteocalcin (OC) was up regulated on HA/PLLAcomposite by RT-PCR analysis. The preliminary study suggested that the use of the controlledmodification of hydroxyapatite coating on PLLA scaffold to produce HA/PLLA composite mightenhance cellular activity, indicating the potential use for bone substitute in tissue engineering
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.707.pdf