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  • 1
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Treatment of aliergic asthma with inhaled corticosteroids, such as budesonide (BDN), results in downregulation of T-cell activation and of eosinophil recruitment.Objective Since blood concentrations of BDN, although significantly lower than those measured in the lung, may still have anti-inflammatory effects, we evaluated the activity of BDN in vitro on: allergen-induced release of lymphokines involved in eosinophil chemotaxis (i.e. IL-3 and IL-5), at drug concentrations similar to those obtained in vivo in the lung (10−8 M), and eosinophil locomotion, at ‘systemic concentrations’ of the drug (10−10 M and 10−9M).Methods Twenty-three atopic asthmatic subjects (atopics) sensitized to Dermatophagoides pteronyssinus (Dp) and seven non-atopic healthy subjects (controls) were studied. Purified blood mononuclear cells (BMC) were stimulated with Dp, with or without BDN 10−8 M and, after 6 days, the supernatants were collected and frozen to test their ehemotactie activity toward purified blood eosinophils and their levels of interleukin (IL)-3 and IL-5 by immunoassay. BMC were then pulsed for additional 18h with [3H]thymidine to evaluate allergen-induced T-cell proliferation. In addition, to test possible direct effects of ‘systemic concentrations’ of the drug on eosinophil locomotion, blood eosinophils were incubated for 1 h with BDN (10−10 M and 10−9 M) prior to test their ehemotactie response toward recombinant human IL-3 and IL-5.Results Stimulation of BMC from atopies with Dp induced a statistically significant increase in [H]thymidine incorporation (P 〈 0.05); secretion of ehemotactie factors for eosinophils (P 〈 0.001) and the release of IL-3 and IL-5 (P 〈 0.005 and P 〈 0.05 respectively). BDN, at the concentration of 10−8 M, was able to significantly down-regulate T-cell proliferation (P 〈 0.05), the secretion of ehemotactie factors for eosinophils (P 〈 0.001) and the release of IL-3 and IL-5 (P 〈 0.01 and P 〈 0.05 respectively). Similarly, “systemic concentrations” of BDN (10−10 M and 10−9 M) totally inhibited the ehemotactie response of blood eosinophils toward recombinant human IL-3 and IL-5 (P 〈 0.005).Conclusions Concentrations of BDN similar to those obtained in vivo are effective in inhibiting both the release of eosinophils chemotaxins by allergen-activated mononuclear cells and eosinophil locomotion.
    Type of Medium: Electronic Resource
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