ISSN:
1600-0714
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Background: Marsupialization results in the reduction of odontogenic cyst size. Interleukin-1α (IL-1α) is thought to play a crucial role for the expansion of odontogenic keratocysts. The aim of this study was to investigate the effects of marsupialization on the expression of IL-1α and on the proliferating activity of a lining epithelium in odontogenic keratocysts.Methods: The concentrations of IL-1α, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the intracystic fluids of odontogenic keratocysts were measured by the enzyme-linked immunosorbent assay (ELISA). The expression of IL-1α mRNA in odontogenic keratocysts was measured before and after marsupialization by in situ hybridization. The expression of IL-1α and epithelial cell-proliferating activities in odontogenic keratocysts were also measured by immunohistochemistry using antibodies for human IL-1α and Ki-67 antigen, respectively.Results: The intracystic fluid levels of IL-1α were significantly higher than those of IL-6 and TNF-α in odontogenic keratocysts. In situ hybridization and immunohistochemistry showed that strong expression of IL-1α mRNA and protein was mainly detected in the epithelial cells of odontogenic keratocysts. After marsupialization, the signal intensities for IL-1α mRNA and protein were significantly decreased. In addition, the Ki-67 labeling index of the epithelial cells was decreased proportionally with the grade of IL-1α mRNA expression after the marsupialization.Conclusion: Our findings suggest that marsupialization may reduce the size of odontogenic keratocyst by inhibiting IL-1α expression and the epithelial cell proliferation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1034/j.1600-0714.2002.00029.x