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  • Articles: DFG German National Licenses  (2)
  • Electronic Resource  (2)
  • 2000-2004  (2)
  • 1975-1979
  • Dendranthema grandiflorum (Ramat.) Kitamura  (1)
  • Gracilis myocutaneous flap  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International journal of clinical oncology 5 (2000), S. 341-344 
    ISSN: 1437-7772
    Keywords: Key words Vesico-vaginal fistula ; Radiation ; Cervical cancer ; Gracilis myocutaneous flap ; Reconstruction surgery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Surgical repair of a radiation-induced vesico-vaginal fistula by a simple closure technique is extremely difficult. Here, we report a patient with a radiation-induced vesico-vaginal fistula that was successfully reconstructed using a gracilis myocutaneous flap. The vesico-vaginal fistula developed in an 83-year-old Japanese woman who had undergone a radical hysterectomy for cervical cancer of the uterus at age 63, and had pelvic radiation therapy for recurrent disease 17 years after the surgery. The fistula was repaired by a reconstruction technique that employed a gracilis myocutaneous flap. Two-thirds of the vaginal mucosa on the side where the fistula was present was completely removed to obtain a wide mucosal defect. Irradiated fibrous tissue around the fistula was removed, and the bladder mucosa was mobilized, and then closed with absorbable sutures. An island gracilis myocutaneous flap was designed on the medial aspect of the patient's left thigh. The first incision was made through the proximal outline of the flap, and its vascular pedicle, the medial circumflex femoral vessels, was dissected. After a complete incision through the flap, the skin flap, including the entire gracilis muscle, was elevated with the pedicle vessel. The distal portion of the myocutaneous flap was then turned to overlay the mucosal defects on the vagina. Postoperatively, there was no flap necrosis and no infection in the vaginal cavity. Indwelling catheterization was required for 8 weeks. Retrograde cystography confirmed complete closure of the fistula.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Key words Chrysanthemum ; Dendranthema grandiflorum (Ramat.) Kitamura ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Somatic embryogenesis was observed in ray-floret explants of Dendranthema grandiflorum (Ramat.) Kitamura cv. Aboukyu on Murashige and Skoog medium containing high concentrations of 3-indoleacetic acid (IAA) and kinetin. 1-Naphthaleneacetic acid also induced somatic embryogenesis but indole-3-butyric acid or 2,4-dichlorophenoxy acetic acid did not. Other cytokinins, such as 6-benzylaminopurine (BAP) and thidiazuron, were also not effective. No embryos were seen at lower IAA concentrations with kinetin and various concentrations of BAP, although higher BAP concentrations yielded many adventitious shoots. In contrast, no somatic embryogenesis was observed from leaves using any combination of plant growth regulators. Histologically, primordia showed a typical embryo shape with a well-developed vascular bundle between the shoot and the root primordia. Embryos had both stomata cells and a root system with polarity. Plants were efficiently regenerated from ray floret-derived embryos subcultured in the appropriate medium.
    Type of Medium: Electronic Resource
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