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  • Artikel: DFG Deutsche Nationallizenzen  (3)
  • Digitale Medien  (3)
  • CP4/GOX  (1)
  • Controlled release fertilizers  (1)
  • DNA polymorphism  (1)
Datenquelle
  • Artikel: DFG Deutsche Nationallizenzen  (3)
Materialart
  • Digitale Medien  (3)
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 34 (1993), S. 121-126 
    ISSN: 1573-0867
    Schlagwort(e): Controlled release fertilizers ; electroultrafiltration ; nitrogen ; ryegrass
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The efficiency of different nitrogenous fertilizers under forced leaching conditions was determined in pot experiments using ryegrass as an indicator plant in a sandy loam soil. Treatments were: ON (unfertilized), AN (ammonium nitrate), U (urea), DAP (diammonium phosphate), T (Triabon), FK (Floranid Komplett) and CDU (crotonylidendiurea). N source effects on N uptake, and N leached (as % of N applied) were evaluated. Results show that the fertilizers which behaved best with regard to N uptake were FK followed by Triabon and AN. The most soluble fertilizers, AN and urea, gave the highest N leaching losses. Significant correlations between EUF-NI (rapidly soluble fraction) and leaching, as well as between EUF-N (I + II) and N uptake by the plant (where EUF-N II relates to the slowly soluble fraction) were obtained.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1432-203X
    Schlagwort(e): Wheat ; transformation ; glyphosate ; CP4/GOX ; embryos
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9–12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1437-1596
    Schlagwort(e): PM loci (LDLR, GYPA, HBGG, D7S8, GC) ; PCR ; DNA polymorphism ; Population genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract Allele and genotype frequencies for the five loci LDLR, GYPA, HBGG, D7S8 and GC were determined for 374 unrelated Italians using a multiplex PCR-amplification and typing commercial kit. The distribution of the genotype frequencies showed no deviations from Hardy-Weinberg expectations. The combined power of discrimination and chance of exclusion for all five loci were 0.999 and 0.702, respectively. A test for homogeneity was performed and no significant differences were observed among the Caucasian population samples.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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