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  • Articles: DFG German National Licenses  (19)
  • Electronic Resource  (19)
  • 11
    Electronic Resource
    Electronic Resource
    Scanning tunneling microscopy on self-assembled calix[4]resorcinarene monolayer adsorbates on Au(111) (2000)
    Springer
    Applied physics 70 (2000), S. 607-611 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 61.16.Ch; 81.05.Lg; 82.80.Pv
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract. Self-assembled Monolayers of calix[4]resorcinarene receptor molecules on Au(111) were studied by UHV scanning tunneling microscopy and X-ray photoelectron spectroscopy. Highly ordered monolayers were observed with domains oriented at an angle of 60° relative to each other. Molecularly resolved images were investigated and lattice constants found which depended on the preparation solvent. The STM images of two samples, one prepared in 1 mM chloroform/ethyl alcohol adsorbate solution and one in 1 mM hexane adsorbate solution are consistent with having a ×2 and 4×2 lattice, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390050001
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  • 12
    Electronic Resource
    Electronic Resource
    Topographical distribution of coated pits (1980)
    [s.l.] : Nature Publishing Group
    Nature 286 (1980), S. 727-729 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Coated pits were defined as regions of membrane indentation approximately 0.3 ?? long and underlain by a fuzzy coat which in favourable micrographs appeared as periodic spikes or bristles (Fig. 1). They frequently occupied the bases of microvilli and were characteristically separated by a clear ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/286727a0
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  • 13
    Electronic Resource
    Electronic Resource
    Reno-cerebral oxalosis induced by xylitol (1984)
    Springer
    Neuroradiology 26 (1984), S. 517-521 
    Details
    ISSN: 1432-1920
    Keywords: Xylitol-infusions ; calcium oxalate crystals in reno-cerebral vessel walls ; perivascular inflammatory reactions and brain edema ; computed tomography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 20-year-old man suffering from Crohn's disease developed coma and generalized seizures following ileocecal resection. During postoperative parenteral feeding he received xylitol in an unusually high concentration. CT examinations a few days before death showed intense hypodensity and swelling of brainstem and basal ganglia and increasing triventricular dilatation. Autopsy revealed, mainly in the brainstem and cerebellum, a destruction of intracerebral, intracerebellar and leptomeningeal vessel walls by birefringent crystals (probably calcium oxalate), an early inflammatory reaction and severe brain edema with final tonsillar herniation. The same crystalloid deposits were found in the kidneys.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00342692
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  • 14
    Electronic Resource
    Electronic Resource
    Isolation and separation of toad bladder epithelial cells (1979)
    Springer
    The journal of membrane biology 48 (1979), S. 141-166 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The epithelium of the urinary bladder ofBufo marinus is composed of 5 cell types, i.e., granular (Gr), mitochondria-rich (MR) and goblet (G) cells which face the urinary lumen, microfilament-rich (MFR) and undifferentiated cells (Un) located basally. The epithelium was dissociated by collagenase and EGTA treatment. Fractionation of dispersed cells by isopycnic centrifugation on dense serum albumin solutions yielded 4 fractions: (i) a very light fraction ( $$\bar \rho \simeq 1.025$$ ) enriched in MR and MFR cells; (ii) a light fraction ( $$\bar \rho \simeq 1.045$$ ) enriched in vacuolated Gr cells; (iii) a heavy fraction ( $$\bar \rho \simeq 1.065$$ ) composed essentially of aggregated Gr cells, and (iv) a pellet ( $$\bar \rho \simeq 1.085$$ ) enriched in G and undifferentiated cells. Recoveries were based on cell counts and DNA measurements. DNA content per cell was 13.2 pg±0.9 (n=37). From 1 g fresh tissue, 62±5×106 (n=10) cells were recovered before isopycnic centrifugation of which about 70% excluded Trypan blue. After centrifugation, 90 to 95% of the cells excluded the vital dye and ∼39×106 cells were recovered from the gradient. Cell metabolism in each fraction was estimated by oxygen consumption measurements in absence or presence of ouabain, acetazolamide, and dinitrophenol. The consumption was threefold higher in the very light and light fractions when compared to the heavy and pellet fractions. Ouabain sensitive oxygen consumption (QO2) represented 12 to 35% of the total O2 consumption depending on the cell fraction, and acetazolamide sensitive QO2 varied from −0.8% in the heavy fractions to 20% in the lighter fractions. DNP increased QO2 in all fractions by 20 to 50%. Finally, the cells were able to reaggregate and form junctional complexes upon addition of calcium to the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872856
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  • 15
    Electronic Resource
    Electronic Resource
    Microfilament-rich cells in the toad bladder epithelium (1979)
    Springer
    The journal of membrane biology 48 (1979), S. 167-180 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Basal cells of the bladder epithelium ofBufo marinus have been found heterogenous and consist of microfilament-rich cells (MFR-cell) and undifferentiated cells (Un-cell). The MFR-cell, which represents approximately 20% of the epithelial cell population, lies between the epithelial layer lining the urinary space and the basement membrane; it extends under several epithelial cells by processes of varying widths and lengths which contact, via desmosomes, other MFR-cells, as well as cells in the superficial layer, i.e., granular and mitochondria-rich cells. The cytoplasm of MFR-cell is filled with intermediate filaments arranged in bundles which run parallel to the plane of the epithelium and no dense granules, typical of granular cells, have been detected. Strong immunofluorescence for actin is associated with cells which occupy the same basal position as MFR-cells. Undifferentiated cells have no contact via desmosomes with adjacent cells and their cytoplasm is filled with free ribosomes; they lack bundles of intermediate filaments and posses no specialized organelles. After a 4-hr pulse of3H-thymidine, 1.5% of epithelial cells incorporate thymidine into nuclear DNA, out of which 3/4 are basally 1/4 are apically located. Identification of cell types by electron microscopy reveals that ∼10% of undifferentiated basal cells are labeled, whereas less than 0.1% of granular cells and no MFR-cells incorporate3H-thymidine into DNA. When dissociated from the epithelium and separated by isopycnic centrifugation, MFR-cells possess a mean buoyant density of approximately 1.025, cosediment with mitochondria-rich cells and exhibit a strong immunofluorescence for actin. The function of MFR-cells remains unknown; however, they may play a role in cell coupling and responses to hormonal and physical factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872857
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  • 16
    Electronic Resource
    Electronic Resource
    Betrachtungen über die rheologischen Eigenschaften von Asphaltbitumina und Arbeitshypothesen über die innere Struktur dieser Produkte (1936)
    Springer
    Colloid & polymer science 76 (1936), S. 95-111 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01432467
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  • 17
    Electronic Resource
    Electronic Resource
    Beiträge zur Kenntnis der thermischen Grundlagen der Verschwelung und Verkokung von Braunkohlen (Auszug) (1935)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 48 (1935), S. 17-21 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19350480107
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  • 18
    Electronic Resource
    Electronic Resource
    Mapping gold-labeled receptors on cell surfaces by backscattered electron imaging and digital image analysis: Studies of the IgE receptor on mast cells (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 185 (1989), S. 128-141 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The response of cells to signalling molecules such as hormones, growth factors, and immune mediators that bind to cell-surface receptors depends in part on the density and distribution of the relevant receptors. We have developed methods to map the distribution of IgE receptors on RBL-2H3 mast cells at high resolution in the scanning electron microscope (SEM). The key elements of our procedure are a new fixative that preserves receptor binding activity; a family of colloidal gold-conjugated probes that bind directly or indirectly to the IgE-receptor complex; an SEM with detectors for both secondary and backscattered electrons (to observe surface topography and gold particles, respectively); and an image processor that can average, digitize, and store these images. Topographical maps are generated by processing and superimposing the digitized images. The methods we describe can be applied to study the density and distribution of any membrane receptor that can be labeled with colloidal gold particles.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001850206
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  • 19
    Electronic Resource
    Electronic Resource
    Aza- und Thia-2-germa(II)-indane und entsprechende 2,2′-Spirobi(2-germaindane) (1989)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 572 (1989), S. 145-150 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Aza- and Thia-2-germa(II)-indanes and Corresponding 2,2′-Spirobi(2-germaindanes)Reactions of dilithiated 1,2-phenylenediamine, N,N′-dimethylphenylenediamine, 1,2-dimercaptobenzene, 3,4-dimercaptotoluene and N-methyl-2-aminothiophenole with the dioxane complex of germanium dichloride give cyclic germylenes of the germaindane type (1-5). Corresponding 2,2′-spirobi(2-germaindanes) (6-9) are obtained as byproducts (by thermal treatment). The compounds are characterized by elemental analyses and by the mass- and n.m.r. (1H, 13C) spectra. A x-ray structure analysis has been performed for 7.
    Notes: Umsetzungen von dilithiiertem 1,2-Phenylendiamin, N,N′-Dimethylphenylendiamin, 1,2-Dimercaptobenzol, 3,4-Dimercaptotoluol und N-Methyl-2-aminothiophenol mit dem Germaniumdichlorid-Dioxan-Komplex führen zu cyclischen Germylenen vom Germaindantyp (1-5). Als Nebenprodukte (durch Erhitzen) entstehen aus diesen Germylenen entsprechende 2,2′-Spirobi(2-germaindane) (6-9). Die Verbindungen sind analytisch sowie Massen- und NMR (1H, 13C) spektroskopisch charakterisiert. Von 7 liegt eine Röntgenstrukturanalyse vor.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19895720118
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