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  • Articles: DFG German National Licenses  (5)
  • 1990-1994  (3)
  • 1985-1989  (2)
  • 1991  (3)
  • 1985  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Detection of herpes simplex viral DNA in Kaposi's varicelliform eruption using in situ hybridization method (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental dermatology 16 (1991), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Herpes simplex viral DNA was detected in biopsy specimens obtained from four patients with Kaposi's varicelliform eruption using an in situ hybridization technique with btotinylated complementary DNA probes. Herpes simplex viral DNA was consistently found almost exclusively in the nuclei of giant cells and balloon cells in vesicles during day 2, 5 and 7 of the disease. In the nuclei, the viral DNA staining pattern was granular or agglomerate. No positive staining was observed in the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2230.1991.tb01223.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Application of immunoblotting to serum protein phenotyping with reference to α2HS-glycoprotein (AHS) typing of bloodstains (1985)
    Springer
    International journal of legal medicine 95 (1985), S. 153-158 
    Details
    ISSN: 1437-1596
    Keywords: Serum groups, immunoblotting ; α2HS-glycoprotein typing ; Bloodstains, immunoblotting ; Serumgruppen, Immunoblottierung ; α2HS-Glykoprotein, Typisierung ; Blutspuren, Immunoblottierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Seren und Blutextrakte wurden auf Polyacrylamidgelen isoelektrisch fokussiert. Die fokussierten Proteine wurden auf Nitrozellulosemembranen durch Diffusion oder elektrophoretisch transferiert. Durch ein spezifisches Antiserum erfolgte auf den Membranen die AG-AK-Reaktion. Nach Waschen der Membranen wurde der gebundene Antikörper mit einem enzymisch markierten Anti-Kaninchen IgG-Antikörper nachgewiesen. Mit dieser Technik konnten die gruppenspezifische Komponente, das α2HS-Glykoprotein, die Komplementkomponenten C6 und C7, der Gerinnungsfaktor 13B und das Plasminogen sehr empfindlich phänotypisiert werden. Vorzugsweise konnten bis zu 6 Monate alte Blutspuren richtig typisiert werden.
    Notes: Summary Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, α2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for α2HS-glycoprotein by the blotting technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201072
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrasound examination of caesarean section scars during pregnancy (1991)
    Springer
    Archives of gynecology and obstetrics 248 (1991), S. 129-138 
    Details
    ISSN: 1432-0711
    Keywords: Ultrasound ; Caesarean section scar ; Conventional method ; New method ; Lower uterine segment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two hundred and sixteen transverse caesarean section scars were examined sonographically near term by a conventional method (175 scars) and a new method (41 scars). The new method consisted of obtaining a transabdominal longitudinal scan by the conventional method and also by a 3M conductor, a transabdominal frontal scan to give a surface view of the scar, and transperineal and transvaginal longitudinal scans. The new method was used from 16 weeks of gestation onwards. Of 41 scars scanned by the new method, 31 showed good healing, being more than 2 mm in thickness throughout; 10 scars showed poor healing with a thickness of less than 2 mm and loss of continuity. Of 31 patients with good healing, 8 delivered vaginally and the remaining 23 patients had repeat caesarean sections for other obstetric indications. All patients with ultrasound evidence of poor healing had repeat caesarean sections. At operation the thickness of the lower uterine segment was measured with ophthalmic calipers. There were 4 false negative results (4/83: 4.8%) and 1 false positive result (1/43: 2.3%) with conventional ultrasound and no false positives or false negatives with the new method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02390090
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cell proliferation and cell loss in intramucosal signet ring cell carcinoma of canine stomachs induced by N-ethyl-N′-nitro-N-nitrosoguanidine (1985)
    Springer
    Journal of cancer research and clinical oncology 110 (1985), S. 87-94 
    Details
    ISSN: 1432-1335
    Keywords: Stomach ; Signet ring cell carcinoma ; Cell turnover ; Tritiated thymidine autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Signet ring cell carcinoma was induced in canine stomachs by N-ethyl-N′-nitro-N-nitrosoguanidine, and modes of cell proliferation and turnover in the carcinoma were studied by 3H-thymidine autoradiography in conjunction with morphometric analysis. From 2 to 15 months after the cessation of 8 months carcinogen treatment, carcinomas in an early stage were obtained. Most of the cancer tissues confined to the lamina propria showed a layered structure. This comprised three layers; the superficial and the deep layer were composed of signet ring cells, and the middle layer was composed of small round cells. The dogs were labeled with 3H-thymidine by s.c. injection and by local infusion of the celiac artery. Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Using a pulse labeling experiment, those labeled carcinoma cells were shown to migrate from the middle layer towards the surface. Morphometric analysis of the autoradiographs showed that the small cells in the middle layer migrated upwards and produced mucin to become full-blown signet ring cells by 5.5 days. In 15 days, most labeled cancer cells in the superficial layer had disappeared. This mode of cellular turnover appeared to mimic a cell renewal system of the normal gastric mucosa. If the cancer cells turn over in this way, the tumor must grow slowly, remaining as an intramucosal cancer for a relatively long period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402717
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Morphology and modes of cell proliferation in earliest signet-ring-cell carcinomas induced in canine stomachs byN-ethyl-N′-nitro-N- nitrosoguanidine (1991)
    Springer
    Journal of cancer research and clinical oncology 117 (1991), S. 197-204 
    Details
    ISSN: 1432-1335
    Keywords: Stomach ; Signet-ring-cell carcinoma ; Cell kinetics ; Bromodeoxyuridine ; N-ethyl-N′-nitro-N-nitrosoguanidine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Signet-ring-cell carcinomas were induced in the stomach of 12 beagle dogs by p.o. administration ofN-ethyl-N′-nitro-N-nitrosoguanidine (ENNG), and the morphology and modes of cell proliferation in an incipient stage of cancer growth were studied with bromodeoxyuridine (BrdUrd) incorporation. From 5 to 27 months after the completion of 8 months' carcinogen treatment, minute carcinomas were found in the stomachs of 9 dogs. Before sacrifice, the dogs were given a single or repeated i.v. injections of BrdUrd for 1–3 days. Minute signet-ring-cell carcinomas were found to form a layered structure, in which the cancer cells proliferated in the lamina propria at the gland-neck level and differentiated to postmitotic signet-ring cells at the upper and lower levels of the mucosa. From repeated injections of BrdUrd, the time required for all the proliferative cells to be labelled with BrdUrd (reflecting the maximum cellcycle time) was estimated to be 1.7 days for the normal glands, and 2.7 days for minute signet-ring-cell carcinomas. From the labelling index with BrdUrd as well as from the morphology, earliest carcinomas were identified in the single gland. There remained atrophic normal epithelium commonly in the single-gland lesions. Proliferative atypical cells appeared to be shed into the stroma passively through the atrophy and subsequent collapse of the gland rather than through active invasion. This may be a reason why cancer cells in minute signet-ring cell carcinomas preserved the normal pattern of cell renewal movement to form the layered structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01625425
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    Paper (German National Licenses)
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