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  • 1
    Electronic Resource
    Electronic Resource
    Clinical pathology of primitive gliomas in the cerebrum (1986)
    Springer
    Acta neurochirurgica 81 (1986), S. 94-99 
    Details
    ISSN: 0942-0940
    Keywords: Primitive glioma ; ependymoblastoma ; undifferentiated glioma ; unclassified glioma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To clarify the biological features of primitive gliomas in the cerebrum and clearly distinguish them from malignant or anaplastic gliomas and glioblastomas, we studied eight cases clinically and pathologically. Our evaluations included immunohistochemical and electron microscopic observations. We divided the patients into two groups, children and young adults. Most tumours appeared as ring-like, enhanced masses on computed tomography and avascular or ring-like, vascular masses on angiography. Macroscopically, the tumours were well demarcated and contained cysts. Ocassionally we found tumour dissemination. Microscopically, the tumours were composed of small, round cells without remarkable structural features. Ependymal, astroglial, and oligodendroglial differentiation was evident, in varying proportions; tumours in which the differentiated areas constituted more than half of the mass were classified as poorly differentiated gliomas. By these criteria, this series comprised four undifferentiated and four poorly differentiated gliomas. Cell anaplasia and polymorphism were rare in both undifferentiated and differentiated areas of the tumours. Immunohistochemical and electron microscopic examinations also revealed glial differentiation. These primitive gliomas appear to be biologically similar, but not identical, to cerebellar medulloblastomas. In this series, five patients died because of recurrence or dissemination. Whole brain and spinal irradiation should be considered after total or subtotal surgical removal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01401228
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  • 2
    Electronic Resource
    Electronic Resource
    High level radioactive nuclides in Japan in May (1986)
    [s.l.] : Nature Publishing Group
    Nature 321 (1986), S. 819-820 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-The accidental release of radioactive materials from the Chernobyl nuclear power plant on 26 April 1986 is known to have caused significant pollution in Western Europe at distances as great as 2,000 km. We have been measuring natural and artificial radionuclides in the environment of Japan for ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/321819a0
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  • 3
    Electronic Resource
    Electronic Resource
    Specific determination of arylsulfatase A activity (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 33-35 
    Details
    ISSN: 1420-9071
    Keywords: Arylsulfatase A ; natural substrate ; ascorbate-2-sulfate ; HPLC-amperometric detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01975881
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of fetal hemoglobin in blood stains by high performance liquid chromatography (1989)
    Springer
    International journal of legal medicine 102 (1989), S. 437-444 
    Details
    ISSN: 1437-1596
    Keywords: Fetal hemoglobin, identification in blood stains ; Blood stains, identification of fetal hemoglobin ; Fetales Hämoglobin, in Blutspuren ; Blutspuren, Nachweis von fetalem Hämoglobin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wurde eine neue Methode zur Identifizierung von fetalem Hämoglobin (Hb F) in Blutspuren mit der umgekehrten Phase-Hochleistungsflüssigkeitschromatographie durchgeführt. Die Unterscheidung zwischen fetalen Blutspuren und denen von Erwachsenen erfolgte aufgrund der Existenz der Spitzen der γ-Ketten von Hb F. In den Chromatogrammen der untersuchten Blutspuren von Erwachsenen traten fast keine γ-Ketten auf. Diese Methode ermöglichte ferner die Bestimmung des Hb F-Wertes mit dem Flächenverhältnis der Globin-Ketten auf den Chromatogrammen. Hb F-Werte der sechs Fälle von Nabelschnurblutspuren auf dem Filterpapier lagen zwischen 81.1% und 91.3%. Die vorliegende Untersuchung zeigt, daß sich der Hb F-Wert für mindestens 12 Wochen nicht ändert. In der Praxis der Rechtsmedizin ist diese Methode im Hinblick auf die Einfachheit, hohe Empfindlichkeit, Schnelligkeit und Zuverlässigkeit sehr wertvoll.
    Notes: Summary A new method for the identification of fetal hemoglobin (Hb F) in blood stains by reverse-phase high performance liquid chromatography is described. Differentiation between fetal and adult blood stains is based on the existence of γ-chain peaks which are characteristic of Hb F. Very few γ chains appeared on chromatograms of all the adult blood stains examined. The level of Hb F could be determined by measuring the total of chromatogram γ-globin chain areas, and expressing it as a percentage of total Hb. Levels in six cord blood stains on filter paper ranged from 81.1% to 91.3% and remained constant for at least 12 weeks. This method is of great value for its simplicity, sensitivity and speed, and most importantly for its reliability in the field of forensic medicine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204074
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  • 5
    Electronic Resource
    Electronic Resource
    Mössbauer sprectroscopic characterization of iron chlorophyllins (1986)
    Springer
    Hyperfine interactions 29 (1986), S. 1403-1406 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The Mössbauer spectra of bis-adducts of pyridine (py) and its derivatives (4-Xpy, where X is CN, CHO, COOCH3, COCH3 and CH3) have revealed that the axial coordination of py and 4-Xpy to iron (III) chlorophyllin gives rise to spontaneous reduction of the central iron (III) ion. A linear correlation between isomer shifts and quadrupole splittings is explained on the basis of the predominant σ-donation from the axial ligand to the central iron ion. The linear dependence of the isomer shifts on the Hammett substituent constants has demonstrated that the larger the isomer shift, the stronger the electron-attracting power of the substituent at the 4-position. The decrease in the quadrupole splitting is ascribed to the relative decrease in the electron density accumulated in the equatorial direction through the σ-donation. The general tendency in the spontaneous reduction of iron (III) chlorophyllin is interpreted in terms of the σ-donor and π-acceptor power of the axial ligands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399496
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of Prussian blue and its thermal decomposition products (1989)
    Springer
    Hyperfine interactions 46 (1989), S. 723-731 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The mixed-valence state of Prussian blue and its thermal decomposition products has been studied by Mössbauer and infrared spectra, and X-ray powder diffraction and conductivity measurements. The57Fe Mössbauer spectra have revealed that the coordination environment of Prussian blue is not changed by the heat-treatment at lower than 200°C while the flipping of the cyano ligands takes place when the heat-treatment temperature exceeds 250°C. The electrical conductivity of the Prussian blue samples heat-treated in vacuo at 300 and 350°C is higher than that of the samples heat-treated at lower temperatures. All the spectral measurements have demonstrated that a new mixed-valence state is produced in Prussian blue by thermally flipping the cyano ligand and quenching the flipped cyano ligand to liquid nitrogen temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02398265
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  • 7
    Electronic Resource
    Electronic Resource
    Mössbauer spectroscopic and magnetic properties of iron/III/ pheophytins (1989)
    Springer
    Journal of radioanalytical and nuclear chemistry 136 (1989), S. 159-168 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Mössbauer spectra of /pheophytinato a/ iron/III/ chloride [Fe/Pheo-a/Cl] and /pheophytinato b/ iron/III/ chloride [Fe/Pheo-b/Cl] have revealed that the central iron/III/ ion is in a high-spin state and axially coordinated by chloride ions in solid phase. Temperature-dependent asymmetry observed for the quadrupole splitting doublet of iron/III/ pheophytins, i.e., Fe/Pheo-a/Cl and Fe/Pheo-b/Cl, is interpreted on the basis of the zero-field splitting. The structural difference between iron/III/ pheophytins and iron/III/ porphyrins is reflected in the quadrupole splitting more than in the isomer shift. Magnetic behaviour of iron/III/ pheophytins near 4.2 K is very similar to that of iron/III/ porphyrins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02164106
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  • 8
    Electronic Resource
    Electronic Resource
    Mössbauer study of iron/III/ pyropheophytins (1989)
    Springer
    Journal of radioanalytical and nuclear chemistry 137 (1989), S. 277-286 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract /Pyropheophytinato a/ iron/III/ chloride [Fe/Pyropheo-a/Cl] and /pyropheophytinato b/ iron/III/ chloride [Fe/Pyropheo-b/Cl] have been prepared and characterized by Mössbauer spectroscopy. The Mössbauer parameters of the iron/III/pyropheophytins are similar to those of iron/III/ porphyrins. A comparison of the quadrupole splittings for the iron/III/ pyropheophytins with those for the iron/III/ pheophytins suggests that the deviations of the cyclopentenone ring and the saturated pyrrole ring from the macrocyclic plane become smaller with the removal of the methoxycarbonyl group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02163553
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  • 9
    Electronic Resource
    Electronic Resource
    Separation and determination of nitrite ion by affinity chromatography with cyano(pheophytinato a)cobalt(III) charged column (1989)
    Springer
    Chromatographia 27 (1989), S. 461-464 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlorophyll-bonded stationary phase ; Cyano(pheophytinato a)cobalt(III) ; Pheophytin a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The separation and determination of the nitrite ion by affinity chromatography is described. The good separation of the nitrite ion was obtained by using cyano-(pheophytinato a)cobalt(III) bonded octadecylsilica as the stationary phase and water of pH 4.3 adjusted by acetic acid as the mobile phase. Conventional ion chromatograph was also used for the analysis of the nitrite ion. For the synthetic samples prepared by mixing the nitrite and nitrate ions, analytical values obtained by affinity chromatography were in agreement with calculated values and close to the analytical values obtained by ion chromatography. In the proposed affinity chromatography, the nitrite and nitrate ions could be determined in the concentration range of 0.15–1.0ppm and 0.1–1.0ppm with relative standard deviations (n=10) of 5.3% and 4.7%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02319564
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of organic solvents on the activity of glucoamylase (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 1172-1177 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hydrolyses of maltose, maltotriose, and soluble starch catalyzed by glucoamylase (Asp. Niger) were carried out in the aqueous solutions of methanol, ethanol, ethylene glycol, and 1,4-dioxane at 40°C and at the optimum pH in the respective solutions. By the kinetic analysis based on the subsite model, it was shown that the intrinsic rate constant, Kint, was electrostatically affected by the dielectric constant of the hydroorganic solutions. The affinity of the third subsite, A3, which affects the apparent rate constant, K0, was correlated with the ×Gtr's of maltose and amino acid side chains.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260280807
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