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  • Articles: DFG German National Licenses  (3)
  • 1995-1999
  • 1990-1994  (3)
  • 1993  (3)
  • Analytical Chemistry and Spectroscopy  (1)
  • Dirofilaria immitis  (1)
  • Freeze substitution  (1)
Source
  • Articles: DFG German National Licenses  (3)
Material
Years
  • 1995-1999
  • 1990-1994  (3)
Year
  • 1993  (3)
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Effect of cytochalasin A on actin distribution in the fission yeastSchizosaccharomyces pombe studied by fluorescent and electron microscopy (1993)
    Springer
    Protoplasma 176 (1993), S. 24-32 
    Details
    ISSN: 1615-6102
    Keywords: Actin ; Cytoskeleton ; Schizosaccharomyces pombe ; Freeze substitution ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Actin distribution and ultrastructure of the fission yeastSchizosaccharomyces pombe treated with cytochalasin A (CA) were investigated by fluorescence microscopy using rhodamine-conjugated phalloidin (rh-ph) and freeze substitution electron microscopy. Among the cytochalasins tested, CA was most effective and at 5 μg/ ml inhibited the appearance of the actin ring at the cell equator at the stage prior to septum formation and the accumulation of actin dots at the septum-forming site both in wild-type cells and the mutantcdc 11, which is defective in septum formation at restrictive temperature. Freeze substitution electron microscopy of CA-treated cells revealed the displacement and morphological alteration of cytoplasmic vesicles and dictyosomes within 30 min and the appearance of dense bodies in the cytoplasm. A sub-population of cytoplasmic vesicles and dictyosomes were insensitive to CA and maintained their original structure. An electron less dense layer containing filamentous material was noted beneath the plasma membrane and thought to be the area of heavy actin patches stained with rh-ph at the cells ends. These results suggest that CA disrupted an actin network that normally maintains the organization of the secretory pathway involving dictyosomes and vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01378936
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Immunohistological observations on pulmonary tissues from dogs infected withDirofilaria immitis (1993)
    Springer
    Veterinary research communications 17 (1993), S. 109-117 
    Details
    ISSN: 1573-7446
    Keywords: Dirofilaria immitis ; dog ; immunohistology ; lung ; peroxidase-antiperoxidase staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pulmonary tissues from non-infected dogs, naturallyDirofilaria immitis-infected dogs and experimentally infected puppies, selectively necropsied after infection, were assessed using peroxidase-antiperoxidase (PAP) staining technology. Sequential sections of pulmonary tissue were PAP stained with anti-freshD. immitis serum, anti-paraffin processedD. immitis serum, anti-dog immunoglobulin (IgG, IgG Fc, IgM) sera and anti-dog complement (C3) serum, and examined for antibody, complement and forD. immitis antigen. The extent of alveolar septal thickening was positively correlated with infection status. Cellular infiltration was most evident surrounding obstructed areas whereD. immitis werein situ. Antigenic material (microfilariae, eggs, fragmented filariae) labelled by PAP was identified in the pulmonary arteries, alveolar capillaries and alveolar septa. Deposits of complement and IgG, presumably associated with immune complex formation, were also observed in some of the infected dogs. Identification of antigen, antibodies and complement associated with alveolar septal pathology suggested that immune mechanisms were active in its development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839238
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Quantitative applications of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry: Determination of copolymer composition in bacterial copolyesters (1993)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 7 (1993), S. 1033-1036 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Poly(β-hydroxybutyrate-co-β-hydroxyvalerate) of microbial origin has been analyzed by matrix-assisted laser desorption/ionization (MALDI) in a time-of-flight mass spectrometer, after partial methanolysis, using two different instruments, Our results show that the mass spectral intensities recorded in the MALDI mode correlate with previously reported fast-atom bombardment and direct chemical ionization data. MALDI compares favourably with these other two techniques in that it is more suitable to the analysis of complex mixtures and of higher molecular masses.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290071113
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    Paper (German National Licenses)
    Fulltext
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