ISSN:
1435-1536
Keywords:
Key words Poly(D
;
L-lactide)
;
spread monolayer
;
enzymatic hydrolysis
;
cutinase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract The enzymatic hydrolysis of a model poly(D,L-lactide) by cutinase was studied by using a barostat surface balance. A theoretical approach based on the adaptation of the Michaelis–Menten scheme at the interface and a process of random fragmentation of the macromolecules was developed. The role of the interfacial organisation of the reaction products was discussed. Hydrolysis rate constant values and the specific activity were estimated and compared with those obtained for the hydrolysis of lipid monolayers. The process of fragmentation of the interfacial polymer structures was visualised by using AFM imaging.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s003960050103
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