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  • Articles: DFG German National Licenses  (4)
  • 2005-2009  (1)
  • 1985-1989  (3)
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  • Articles: DFG German National Licenses  (4)
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Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor (2005)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 58 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A complex programme of regulation governs gene expression during development of the morphologically and biochemically complex eubacterial genus Streptomyces. Earlier work has suggested a model in which ‘higher level’ pleiotropic regulators activate ‘pathway-specific’ regulators located within chromosomal gene clusters encoding biosynthesis of individual antibiotics. We used mutational analysis and adventitious overexpression of key Streptomyces coelicolor regulators to investigate functional interactions among them. We report here that cluster-situated regulators (CSRs) thought to be pathway-specific can also control other antibiotic biosynthetic gene clusters, and thus have pleiotropic actions. Surprisingly, we also find that CSRs exhibit growth-phase-dependent control over afsR2/afsS, a ‘higher level’ pleiotropic regulatory locus not located within any of the chromosomal gene clusters it targets, and further demonstrate that cross-regulation by CSRs is modulated globally and differentially during the S. coelicolor growth cycle by the RNaseIII homologue AbsB. Our results, which reveal a network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor, suggest that revision of the currently prevalent view of higher-level versus pathway-specific regulation of secondary metabolism in Streptomyces species is warranted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2005.04879.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 26-36 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Promoter-probe plasmid vectors were used to isolate putative promoter-containing DNA fragments of three Streptomyces antibiotic resistance genes, the rRNA methylase (tsr) gene of S. azureus, the aminoglycoside phosphotransferase (aph) gene of S. fradiae, and the viomycin phosphotransferase (vph) gene of S. vinaceus. DNA sequence analysis was carried out for all three of the fragments and for the protein-coding regions of the tsr and vph genes. No sequences resembling typical E. coli promoters or Bacillus vegetatively-expressed promoters were identified. Furthermore, none of the three DNA fragments found to be transcriptionally active in Streptomyces could initiate transcription when introduced into E. coli. An extremely biased codon usage pattern that reflects the high G+C composition of Streptomyces DNA was observed for the protein-coding regions of the tsr and vph genes, and of the previously sequenced aph gene. This pattern enabled delineation of the protein-coding region and identification of the coding strand of the genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327505
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification and characterization of a locus inhibiting extrachromosomal maintenance of theStreptomyces plasmid SLP1 (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 324-331 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid replication ; Incompatibility ; Helix-turn-helix motifs ; Superinfection immunity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report here the existence and initial characterization of a genetic locus (imp) that inhibits maintenance of SLP1-derived plasmids as extrachromosomal replicons in a manner distinct from normal incompatibility between autonomous SLP1 replicons. Thetrans-actingimp function has been localized to a 1.8 kbEco47III restriction fragment present on integrated SLP1 elements. At least part of this DNA segment is absent from SLP1-derived plasmids. DNA sequence analysis of theimp region indicates that it contains three overlapping open reading frames (ORFs) that may constitute a polycistronic operon. The effects of insertions within theimp region indicate that uninterrupted transcription through all three ORFs is necessary forimp activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02464900
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Nocturnal penile tumescence and penile responses in the waking state in diabetic and nondiabetic sexual dysfunctionals (1985)
    Springer
    Archives of sexual behavior 14 (1985), S. 109-129 
    Details
    ISSN: 1573-2800
    Keywords: nocturnal penile tumescence ; sexual arousal ; sexual dysfunction ; impotence ; erectile dysfunction ; sleep ; diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Psychology
    Notes: Abstract This study compared diabetics with sexual dysfunction, nondiabetics with sexual dysfunction, and a group of controls on nocturnal penile tumescence (NPT) during three nights in a sleep laboratory, and penile response to erotic stimulation in the waking state on one of the nights. Both diabetic and nondiabetic dysfunctionals showed less erectile response to erotic films and tape than controls but did not differ from each other. In contrast, the diabetic dysfunctionals showed significantly weaker NPT response than both the nondiabetic dysfunctionals and the controls, and 58% of them (contrasted with 23% of nondiabetic dysfunctionals and 0% of controls) would be classified as organic using minimal NPT (less than 11.5 mm maximal increase in penile circumference during any nocturnal erection) as the sole criterion. There was a significant relationship between NPT and waking erections in response to erotic stimuli, especially in the diabetic dysfunctionals and the controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01541657
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    Paper (German National Licenses)
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