Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of fish biology
37 (1990), S. 0
ISSN:
1095-8649
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A new method for the preparation of fish chromosomes from abdominal cavity fluid has been developed. Cells were collected from fish abdominal cavity fluid after an in vivo PHA treatment, and cultured for a short time in medium with colchicine. After 30 min hypotonic treatment for marine fish and 35 min for freshwater fish, slides were prepared by the conventional air-drying method. The advantages ofthe method are: (1) it is technically simple; (2) it produces a reasonably high mitotic index; (3) chromosome spreading is good (4) there is very little cell breakage. Using this method, the chromosomes ofrainbow trout (2n=62); cod (2n=4546) and plaice (2n=46,47 and 48) were investigated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1095-8649.1990.tb05888.x
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