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  • Articles: DFG German National Licenses  (3)
  • 1995-1999  (3)
  • Affinity chromatography  (1)
  • Cerebral blood flow  (1)
  • Chinese cabbage (Brassica campestris subsp. pekinensis)  (1)
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  • Articles: DFG German National Licenses  (3)
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Years
  • 1995-1999  (3)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Cerebral cortex blood flow and vascular smooth muscle contractility in a rat model of ischemia: a correlative laser Doppler flowmetric and scanning electron microscopic study (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 354-368 
    Details
    ISSN: 1432-0533
    Keywords: Key words Reperfusion ; Cerebral blood flow ; Vascular smooth muscle cell ; Pericyte ; Scanning ; electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was undertaken to ascertain the role of smooth muscles and pericytes in the microcirculation during hyperperfusion and hypoperfusion following ischemia in rats. Paired external carotids, the pterygopalatine branch of the internal carotids and the basilar artery were exposed and divided. Reversible inflatable occluders were placed around the common carotids. After 24 h, the unanesthetized rat underwent 10-min ischemia by inflating the occluders. Continuous cortical cerebral blood flow (c-CBF) was monitored by laser Doppler flowmetry. The measured c-CBF was below 20% of control (P 〈 0.001) during ischemia. A c-CBF of 227.5 ± 54.1% (P 〈 0.001) was obtained during reperfusion hyperemia. A c-CBF of 59.7 ± 8.8% (P 〈 0.001) occurred at the nadir of postischemic hypoperfusion, and this was followed by a second hyperemia. The cytoarchitecture of the vascular smooth muscles and pericytes was assessed by scanning electron microscopy. Samples were prepared using a KOH-collagenase digestion method. In control rats, arteriolar muscle cells showed smooth surfaces. Capillary pericytes were closely apposed to the endothelium. Immediately after reperfusion, transverse membrane creases were observed on the smooth muscle surfaces. During maximal hyperemia the creases disappeared. When c-CBF started to decrease the creases became visible again. Throughout the postischemic hypoperfusion the creases remained. Capillary endothelial walls became tortuous in the late phase of hypoperfusion. During the second hyperemia most arteriolar muscle cells showed smooth surfaces. Some pericytes appeared to have migrated from the vascular wall. The morphological changes of smooth muscle membranes suggest that they are related to specific perfusional disturbances during ischemia and reperfusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050627
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    NADH oxidase ofThermus thermophilus HB8 overproduced fromEscherichia coli (1995)
    Springer
    Protoplasma 184 (1995), S. 104-110 
    Details
    ISSN: 1615-6102
    Keywords: NADH oxidase ; Thermostable flavoprotein ; Affinity chromatography ; Codon usage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An NADH oxidase purified from the extreme thermophileThermus thermophilus HB8 is a monomeric flavoprotein with a 1 ∶ 1 ratio of flavin-adenine dinucleotide (FAD) to the polypeptide chain. It catalyzes in vitro the oxidation of reduced NADH or NADPH with the formation of H2O2. The gene encoding the NADH oxidase fromT. thermophilus HB8 was cloned, and its nucleotide sequence was determined. The molecular mass of 22,749 Da, as deduced from thenox gene, agrees with that of the purified NADH oxidase fromT. thermophilus HB8, as estimated by mass spectrometry. Thenox gene does not contain a GX4GK consensus sequence typical for nucleotide binding proteins. Thenox gene was overexpressed inEscherichia coli, and a protocol for the rapid purification of theE. coli-borneT. thermophilus NADH oxidase or its His6-tagged analogue was developed by using thermal denaturation step and affinity chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276906
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetics of resistance to five strains of turnip mosaic virus in Chinese cabbage (1995)
    Springer
    Euphytica 81 (1995), S. 71-77 
    Details
    ISSN: 1573-5060
    Keywords: Chinese cabbage (Brassica campestris subsp. pekinensis) ; turnip mosaic virus ; resistance ; monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inheritance of resistance to turnip mosaic virus (TuMV) strains C1, C2, C3, C4, and C5 in Chinese cabbage (Brassica campestris subsp. pekinensis) was evaluated using monoclonal antibodies. Crosses were made between a resistant line, ‘0–2’, and four susceptible line. ‘Seoul’ (SE),‘SSD31’ (SS), ‘Cheongbang’ (CH), and ‘Yaki 1 ho’ (YA), to determine the inheritance of resistance of ‘0–2’ in different genetic backgrounds. Resistance to TuMV was controlled by a single dominant gene or double dominant genes depending on the strain and cross. The resistance genes of ‘0–2’ were modified by susceptible parents such that a single dominant gene was involved in the ‘SS×0–2’ combination, but double dominant genes in the ‘SE×0–2’ against TuMV-C3 or TuMV-C5. ELISA tests using inoculated and noninoculated leaves in the same plant suggested that the dominant resistance genes inhibit virus movement rather than virus multiplication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022460
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    Paper (German National Licenses)
    Fulltext
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