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  • Artikel: DFG Deutsche Nationallizenzen  (2)
  • 1995-1999  (2)
  • Affinity chromatography  (1)
  • Cerebral blood flow  (1)
Datenquelle
  • Artikel: DFG Deutsche Nationallizenzen  (2)
Materialart
Erscheinungszeitraum
  • 1995-1999  (2)
Jahr
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Cerebral cortex blood flow and vascular smooth muscle contractility in a rat model of ischemia: a correlative laser Doppler flowmetric and scanning electron microscopic study (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 354-368 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Reperfusion ; Cerebral blood flow ; Vascular smooth muscle cell ; Pericyte ; Scanning ; electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The present study was undertaken to ascertain the role of smooth muscles and pericytes in the microcirculation during hyperperfusion and hypoperfusion following ischemia in rats. Paired external carotids, the pterygopalatine branch of the internal carotids and the basilar artery were exposed and divided. Reversible inflatable occluders were placed around the common carotids. After 24 h, the unanesthetized rat underwent 10-min ischemia by inflating the occluders. Continuous cortical cerebral blood flow (c-CBF) was monitored by laser Doppler flowmetry. The measured c-CBF was below 20% of control (P 〈 0.001) during ischemia. A c-CBF of 227.5 ± 54.1% (P 〈 0.001) was obtained during reperfusion hyperemia. A c-CBF of 59.7 ± 8.8% (P 〈 0.001) occurred at the nadir of postischemic hypoperfusion, and this was followed by a second hyperemia. The cytoarchitecture of the vascular smooth muscles and pericytes was assessed by scanning electron microscopy. Samples were prepared using a KOH-collagenase digestion method. In control rats, arteriolar muscle cells showed smooth surfaces. Capillary pericytes were closely apposed to the endothelium. Immediately after reperfusion, transverse membrane creases were observed on the smooth muscle surfaces. During maximal hyperemia the creases disappeared. When c-CBF started to decrease the creases became visible again. Throughout the postischemic hypoperfusion the creases remained. Capillary endothelial walls became tortuous in the late phase of hypoperfusion. During the second hyperemia most arteriolar muscle cells showed smooth surfaces. Some pericytes appeared to have migrated from the vascular wall. The morphological changes of smooth muscle membranes suggest that they are related to specific perfusional disturbances during ischemia and reperfusion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010050627
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    NADH oxidase ofThermus thermophilus HB8 overproduced fromEscherichia coli (1995)
    Springer
    Protoplasma 184 (1995), S. 104-110 
    Details
    ISSN: 1615-6102
    Schlagwort(e): NADH oxidase ; Thermostable flavoprotein ; Affinity chromatography ; Codon usage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An NADH oxidase purified from the extreme thermophileThermus thermophilus HB8 is a monomeric flavoprotein with a 1 ∶ 1 ratio of flavin-adenine dinucleotide (FAD) to the polypeptide chain. It catalyzes in vitro the oxidation of reduced NADH or NADPH with the formation of H2O2. The gene encoding the NADH oxidase fromT. thermophilus HB8 was cloned, and its nucleotide sequence was determined. The molecular mass of 22,749 Da, as deduced from thenox gene, agrees with that of the purified NADH oxidase fromT. thermophilus HB8, as estimated by mass spectrometry. Thenox gene does not contain a GX4GK consensus sequence typical for nucleotide binding proteins. Thenox gene was overexpressed inEscherichia coli, and a protocol for the rapid purification of theE. coli-borneT. thermophilus NADH oxidase or its His6-tagged analogue was developed by using thermal denaturation step and affinity chromatography.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01276906
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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