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  • Articles: DFG German National Licenses  (2)
  • 1995-1999  (2)
  • Engineering General  (1)
  • Plant microfilaments  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 191 (1996), S. 215-219 
    ISSN: 1615-6102
    Keywords: Laser scanning confocal microscopy ; Multiple cell layers ; Plant microtubules ; Plant microfilaments ; Roots ; Tissue clearing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A protocol was developed to observe plant microtubules and actin microfilaments in large tissue samples without physical sectioning. Rye (Secale cereale L. cv. Rymin) root tip pieces from two-day-old seedlings were fixed and processed for immunolabeling. Incubation times of 24–48 h were required to insure adequate penetration of fixatives, antibodies, and washing buffers. Clearing of the tissue with methyl salicylate reduced background auto-fluorescence that would otherwise interfere with the resolution of cytoskeletal structures. Microtubules or microfilaments in 5–7 cell layers were visualized using the optical-sectioning capability of laser scanning confocal microscopy (LSCM) and projected as three-dimensional images. The three-dimensional character of the cytoskeletal elements is retained when viewing stained cells of intact tissue. The net-like character of a microfilament array radiating out from a single point into the cytoplasm is maintained when the cells are stained in intact root tip pieces and imaging is accomplished in situ.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 20 (1996), S. 331-349 
    ISSN: 0363-9061
    Keywords: elasticity ; buried pipes ; surface heading ; soil-structure interaction ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: A method is presented which may be used to compute the displacements, strains and moments (both in-plane and transverse) in buried structures such as pipelines and culverts subjected to longitudinal bending. This type of bending can occur if a surface loading such as a vehicular loading or an embankment loading is applied to the soil above the pipe or culvert.Fourier transforms are used to reduce the three-dimensional problem to one involving only two spatial directions, thereby reducing the data preparation and computation time. Conventional finite element analysis is used to approximate the field quantities in the transformed two-dimensional plane. Two Fourier integral element types have been developed which have many applications in geotechnical engineering.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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