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  • 1
    Electronic Resource
    Electronic Resource
    Production of diploid and triploid interspecific hybrids between Lilium concolor and L. longiflorum by in vitro ovary slice culture (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Successful hybridization between Lilium concolor and Lilium longiflorum has not been reported but ovary slice culture technique, after cut-style pollination has now been used to produce diploid and triploid interspecific hybrids between these species. Reciprocal crosses between diploid cultivars (2n= 2x= 24) were conducted. 5, 10, 15, 20, 25, 30, 35, 40, and 45 days after pollination (DAP), ovaries were sliced and cultured on a modified Murashige and Skoog (MS) medium without growth regulators and NH4NO3, supplemented with 6% sucrose, 50 mg/1 yeast extract and 0.25% gelrite at pH 6.3. For the L. concolor × L. longiflorum cross, embryo germination was found to be best at 20 DAP, while for the L. longiflorum × L. concolor at 25 DAP. After transfer to a MS (half-strength) medium supplemented with 1.5% sucrose, 0.25% gelrite and 0.2% active charcoal at pH 5.8, diploid and triploid hybrid plants were developed. All regenerated plants were identified as hybrids on the basis of karyotype and isozyme analyses. Ovary slice culture technique as a method of producing polyploids is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1998.tb01977.x
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  • 2
    Electronic Resource
    Electronic Resource
    Development of diploid and triploid interspecific hybrids between Lilium longiflorum and L. concolor by ovary slice culture (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ovary slice culture, after cut-style pollination, was used to develop interspecific hybrids between Lilium longiflorum and L. concolor. Reciprocal crosses between diploid cultivars (2n = 2x = 24) were carried out. On the days 30, 35, 40 and 45th after pollination (DAP), ovaries were sliced and cultured on a modified hormone-free Murashige-Skoog (M–S) medium without NH4NO3, supplemented with 6% sucrose, 50 mg/1 yeast extract and 0.25% gelrite at pH 6.3. For the L. longiflorum × L. concolor cross, ovule germination was found to be best at 30 DAP. After transfer to a M–S (half-strength) medium supplemented with 1.5% sucrose and 0.25% gelrite at pH 5.8, diploid and triploid hybrid plants were established. In contrast, ovules from the L. concolor × L. longiflorum cross did not germinate. The hybridity of the plantlets obtained was verified by karyotype and isozyme analysis. The importance of the ovary slice culture technique as a tool to develop new hybrids between incompatible lilly plants is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1996.tb00895.x
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  • 3
    Electronic Resource
    Electronic Resource
    Neuroprotective actions of peripherally administered insulin-like growth factor I in the injured olivo-cerebellar pathway (1999)
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 11 (1999), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Exogenous administration of insulin-like growth factor I (IGF-I) restores motor function in rats with neurotoxin-induced cerebellar deafferentation. We first determined that endogenous IGFs are directly involved in the recovery process because infusion of an IGF-I receptor antagonist into the lateral ventricle blocks gradual recovery of limb coordination that spontaneously occurs after partial deafferentation of the olivo-cerebellar circuitry. We then analysed mechanisms whereby exogenous IGF-I restores motor function in rats with complete damage of the olivo-cerebellar pathway. Treatment with IGF-I normalized several markers of cell function in the cerebellum, including calbindin, glutamate receptor 1 (GluR1), γ-aminobutyric acid (GABA) and glutamate, which are all depressed after 3-acetylpyridine (3AP)-induced deafferentation. IGF-I also promoted functional reinnervation of the cerebellar cortex by inferior olive (IO) axons. In the IO, increased expression of bax in neurons and bcl-X in astrocytes after 3AP was significantly reduced by IGF-I treatment. On the contrary, IGF-I prevented the decrease in poly-sialic-acid neural cell adhesion molecule (PSA-NCAM) and GAP-43 expression induced by 3AP in IO cells. IGF-I also significantly increased the number of neurons expressing bcl-2 in brainstem areas surrounding the IO. Altogether, these results indicate that subcutaneous IGF-I therapy promotes functional recovery of the olivo-cerebellar pathway by acting at two sites within this circuitry: (i) by modulating death- and plasticity-related proteins in IO neurons; and (ii) by impinging on homeostatic mechanisms leading to normalization of cell function in the cerebellum. These results provide insight into the neuroprotective actions of IGF-I and may be of practical consequence in the design of new therapeutic approaches for neurodegenerative diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9568.1999.00623.x
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  • 4
    Electronic Resource
    Electronic Resource
    Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb.) (1997)
    Springer
    Plant cell reports 16 (1997), S. 836-840 
    Details
    ISSN: 1432-203X
    Keywords: Key words Anther-enriched culture media ; Isozymes ; Lilium longiflorum Thunb ; Lily propagation ; Somaclonal uniformity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bulb scale propagation makes it difficult to obtain a large number of bulblets from disease-free stocks in a short time. The establishment of improved micropropagation procedures by in vitro culture is therefore desirable. Easter lily (Lilium longiflorum Thunb.) filaments with and without anther were excised and cultured in vitro with different media and culture conditions. In cultures of filaments with anther, callus developed and led to bulb, shoot, and root formation, whereas in cultures of filaments lacking anther, callus development did not occur. Among the various media tested, the B5 medium combined with darkness and the N6 medium combined with darkness or light, both supplemented with 9% sucrose, proved to be superior. A total of 1260 plants were regenerated from callus, acclimatized under a mist, and transferred to the greenhouse with a 100% success rate. No morphological abnormalities were observed among plants regenerated from filament-derived callus and all plants displayed isozyme banding patterns identical to the original cultivar. Chromosome observations revealed that all callus-regenerated plantlets tested were diploid (2n=24). The results suggest that in vitro culture of filaments with anther can be cultured for mass propagation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050330
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of the inactivating "ball" peptide of Shaker B on intermediate conductance Ca2+-dependent inwardly rectifying K+ channels of HeLa cells (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 879-882 
    Details
    ISSN: 1432-2013
    Keywords: Intermediate conductance Ca2+-dependent K+ channel Shaker B inactivating peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The patch-clamp technique was used to study the effect of intracellularly added inactivating "ball" peptide (BP) of the Shaker B K+ channel upon Ca2+-dependent inwardly rectifying K+ channels of the intermediate conductance type expressed in HeLa cells. Intracellular BP caused only moderate inhibition of outward K+ currents when assayed at an intracellular Ca2+ concentration of 100 nmol/l. Increasing intracellular Ca2+ levels led in itself to some voltage-dependent blockade of K+ currents, which was absent when high extracellular K+ was used. An additional strong blockade by intracellular BP was nevertheless observed both in Na+- and K+-rich extracellular solutions. A non-inactivating BP analogue had no effect. At this higher intracellular Ca2+ concentration the inhibition of these intermediate conductance Ca2+-dependent channels by BP was voltage-dependent, being absent at hyperpolarizing potentials, and could be relieved by increasing extracellular K+. These data suggest that BP acts at an internal pore site in Ca2+-dependent intermediate conductance K+ channels of HeLa cells, and that these might possess a receptor site for the peptide similar to that of other K+ channels such as Ca2+-activated maxi-K+ channels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900138
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  • 6
    Electronic Resource
    Electronic Resource
    Chemical heterogeneity in adult rat cerebellar purkinje cells as revealed by zebrin I and low-affinity nerve growth factor receptor immunocytochemical expression following injury (1995)
    Springer
    Journal of neurocytology 24 (1995), S. 807-817 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cerebellar Purkinje cells in rat express low-affinity nerve growth factor receptor during development, but rarely in normal adult animals. However, after either mechanical injury or colchicine treatment during adulthood, these cells re-express low-affinity nerve growth factor receptor-immunoreactive protein. Two Purkinje cell subpopulations were defined in normal adult cerebellum by the presence or the absence of zebrin I antigen. Nevertheless, it remains an open question as to whether low-affinity nerve growth factor receptor-immunoreactive protein can be expressed by all damaged Purkinje cells, independent of their location and their staining with antibodies against intrinsic molecular markers that reveal Purkinje cell heterogeneity, such as zebrin I. In this study, a serial-section immunocytochemical mapping of the expression zebrin I and low-affinity nerve growth factor receptor, using specific monoclonal antibodies, was carried out in colchicine-treated rats. After mechanical damage of the cerebellar cortex, co-localization of these antigens at the cellular level was also analysed in thin adjacent sections, and by using a combined immunocytochemical staining method in individual sections. The findings revealed the existence of three sub-sets of Purkinje cells: (1) two complementary groups distinctly immunoreactive to one antibody, but not to the other and (2) a third group that contained double-labelled cells. In contrast, co-expression of both antigens was never observed following mechanical lesions. The seemingly independent response to mechanical injury of Purkinje cells located in different zebrin-defined compartments, indicates that particular subpopulations of Purkinje cells may respond differentially to traumatic injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01179980
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  • 7
    Electronic Resource
    Electronic Resource
    Optimisation of the electrochemical bath for growing device-quality CuInSe2 thin films (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Advanced Materials for Optics and Electronics 8 (1998), S. 1-8 
    Details
    ISSN: 1057-9257
    Keywords: electrodeposition ; copper-indium-selenide ; thin films ; layer-by-layer deposition ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: The electrochemical bath used for growing device-quality CIS (CuInSe2) thin films by co-deposition as well as layer-by-layer (LBL) deposition was characterised and optimised with respect to the film properties. The bath composition was varied by changing the Cu, In and Se ion concentrations in specific ratios in both co-deposition and LBL deposition. The film properties were analysed using techniques such as SEM (scanning electron microscopy), EPMA (electron probe microanalysis), AES (Auger electron spectroscopy) and XRD (X-ray diffraction). The structural, morphological and compositional properties of the films were characterised and their variation is attributed to the bath composition and growth conditions. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Growth modes of solution-grown CdS thin films (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Advanced Materials for Optics and Electronics 5 (1995), S. 11-17 
    Details
    ISSN: 1057-9257
    Keywords: CdS ; chemical deposition ; complexing agent ; sulfurising agent ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: The growth modes of CdS thin films on glass in a chemical bath were analysed using scanning electron microscopy and optical microscopy. The results of these studies show that the film growth occurs by ion-by-ion condensation and by colloidal particles of CdS adhering to the substrate. Both mechanisms are operative from the initial stages of film growth. The predominance of one or other of these two growth modes depends on the abundance of Cd and S ions present in the solution, which is determined by the amount of complexing and sulphurising agents and ammonia used for the controlled release of Cd and S ions into the solution. The growth mode influences the optical properties of the films.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/amo.860050104
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