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  • Articles: DFG German National Licenses  (16)
  • 1995-1999  (16)
  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 49 (1995), S. 277-304 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillan Magazines Ltd.
    Nature 395 (1998), S. 896-900 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The ability to distinguish colour from intensity variations is a difficult computational problem for the visual system because each of the three cone photoreceptor types absorb all wavelengths of light, although their peak sensitivities are at relatively short (S cones), medium (M cones), or ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 22 (1995), S. 0 
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract In this study, we assessed the LFA-1 (CD18/CD11a) and CR3 (CD18/CD11b) expression on peripheral polymorphonuclear leukocytes (PB-PMN) and crevicular fluid polymorphonuclear leukocytes (CF-PMN), by subjects with a healthy periodontium (n=1), gingivitis (n=8). early-onset periodontitis (n=17) and adult periodontitis (n=8). Using flow cytometry analysis, the %s of CD18, CD11a and CD11b positive cells and the absolute numbers of fluorescent molecules were determined. No significant difference could be found among the 4 groups, for these 2 kinds of parameters, in PB-PMN or CF-PMN. However, a great difference could be noted between the results obtained from PB-PMN and those obtained from CF-PMN. The %s of positive CF-PMN were significantly lower than those of PB-PMN for the 3 sub-units (p 〈 0.001). The levels of CD18 and CD11b expressed by CF-PMN were higher than those expressed by PB-PMN and the difference was significant for CD11b (p 〈 0.001). On the contrary, the level of CD11a expressed on CF-PMN was significantly lower than that expressed by PB-PMN (p 〈 0.001). Hence, our current results show that early-onset periodontitis PMN can be quite normal and this fact is not surprising insofar as, in our study, these cells were perfectly functional and all the subjects were in good health. We concluded that the analysis of the leukocyte adhesion receptors expression on PB-PMN does not appear useful for helping to establish a differential diagnosis between the different forms of periodontitis. However, the pattern of expression study on CF-PMN may permit a better comprehension of the local phenomena which are implicated in the defence of the periodontal tissues against oral microorganisms.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using a reverse transcription PCR (RT-PCR) amplification strategy, with degenerate primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, GPX-encoding cDNA fragments were generated from sunflower (Helianthus annuus L.) hypocotyl RNA. Along with the sequences of two distinct GPX-encoding cDNAs (GPXha-1 and GPXha-2) and the alignment of their conceptually translated products with to date cloned plant GPXs, we report here that the two corresponding mRNAs are qualitatively and quantitatively differentially expressed in various organs of H. annuus. Using northern blotting we have shown that more sunflower GPX-like transcripts accumulate in plants which have been subjected to either wounding or infection by a biotrophic pathogen (Plasmopara halstedii) suggesting that the corresponding genes are inducible under various stress conditions.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 134 (1995), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The production of a 25 kDa protein was greatly increased when a sulfur- and ferrous iron-oxidizing species of Sulfolobus was switched from growth on tetrathionate to growth on ferrous iron. The gene encoding the protein was cloned and sequenced. The predicted amino sequence showed significant similarity to those of the alkyl hydroperoxide reductase/thiol specific anti-oxidant family of proteins that appear to be involved in responses to certain types of oxidative stress.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2242
    Keywords: Key words RAPD ; Orobanche cumana ; Helianthus annuus ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The parasitic plant Orobanche cumana Wallr. has become a limiting factor for sunflower crops in infested countries. Over the past few years the progression of this parasitic plant, its introduction into new countries, and the development of new and more virulent races have all been observed. Consequently, the survey and understanding of broomrape population evolution is now crucial for the establishment of efficient breeding programmes. With this in prospect, the genetic variability of O. cumana populations from infested European countries, Bulgaria, Romania, Turkey and Spain, was studied using RAPD markers. Eight populations with a total of 180 plants were analysed. Twenty three primers were used to obtain 133 reproducible bands which led to a binary matrix. This matrix was subjected to various complementary analyses including pairwise distances computed with the Nei and Li coefficient, AMOVA, Nei’s genetic diversity statistics, and an estimation of gene flow among populations with the infinite-island formula. The results gave consistent conclusions whatever the method used for data treatment. We show that this parasitic plant is probably self-pollinated, that there is little intra-population variability, and very little gene exchange appears to occur between different geographic regions. Populations were well structured and organized into two distinct groups (one group corresponding to the East European countries, Bulgaria, Romania and Turkey, and the other group corresponding to Spanish populations) and could have a monophyletic origin. These results are discussed in relation to the applied uses of RAPD markers in the determination of true O. cumana races instead of populations.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2242
    Keywords: RFLP analysis ; Whea ; Aegilops ventricosa ; Leaf rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RFLP analysis has been used to characterise XMv, a chromosome of Aegilops ventricosa present in a disomic addition line of wheat. This chromosome is known to carry a major gene conferring resistance to leaf rust (Lr). The analysis demonstrated that XMv is translocated with respect to the standard wheat genome, and consists of a segment of the short arm of homoeologous group 2 attached to a group 6 chromosome lacking a distal part of the short arm. Lr was located to the region of XMv with homoeology to 2S by analysis of a leaf rust-susceptible deletion line that was found to lack the entire 2S segment. Confirmation and refinement of the location of Lr was obtained by analysis of a spontaneous resistant translocation in which a small part of XMv had been transferred to wheat chromosome 2A.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 1079-1086 
    ISSN: 1432-2242
    Keywords: RFLP ; Helianthus annuus ; Linkage map ; Consensus map ; CDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper provides the first description of a consensus map of the cultivated sunflower genome (Helianthus annuus L., n=17 chromosomes), based on RFLP. A total of 180 probe-enzyme combinations were mapped on at least one of five segregating progenies (three F2 and two BC1 populations), revealing 237 loci that did not show any distortion of segregation. The consensus linkage map obtained with these loci covers 1150 cM and consists of 16 linkage groups of more than 20 cM, 7 groups of less than 20 cM and 18 unlinked loci. The mean distance between loci is 7 cM, but in some regions intervals of 20 cM remain. Genotypic and gametic segregation distortions affect about 7% of loci. It was found that 25% of the probes mapped using several different restriction enzymes or that on different progenies they revealed 2 or more loci.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2242
    Keywords: Helianthus annuus ; Plasmopara halstedii ; Disease resistance ; Bulked segregant analysis ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Pl1 locus in sunflower, Helianthus annuus L., conferring resistance to downy mildew, Plasmopara halstedii, race 1 has been located in linkage group 1 of the consensus RFLP map of the cultivated sunflower. Bulked segregant analyses were used on 135 plants of an F2 progeny from a cross between a downy mildew susceptible line, GH, and RHA266, a line carrying Pl1. Two RFLP markers and one RAPD marker linked to the Pl1 locus have been identified. The RFLP markers are located at 5.6 cM and 7.1 cM on either side of Pl1. The RAPD marker is situated at 43.7 cM from Pl1. The significance and applications of these markers in sunflower breeding are discussed.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2242
    Keywords: Key words Linkage ; Major gene ; Race-specific resistance ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  These studies were undertaken to determine whether downy mildew resistance genes in sunflower were independent as first reported, or linked as suggested by more recent hypotheses. The segregations for downy mildew reaction of 111 F3 progenies from a cross between a susceptible line and a line with Pl2 were used to locate this gene on the sunflower consensus RFLP linkage map. It was shown that Pl2 was linked to the same RFLP markers on linkage group 1 as Pl1 and Pl6, mapped earlier, and at a very similar distance. The F3 progenies showed exactly the same segregation patterns when tested with race 1 and race D. One hundred and fifty four progenies from a cross between a susceptible line and HA335, containing Pl6 (considered as giving resistance to all Plasmopara halstedii races), were tested with the five French downy mildew races, 1, A, B, C and D. Two progenies were observed to show segregation for races 1 and D, while appearing homozygous-resistant to races A , B and C. Tests on F4 progenies confirmed this separation of resistances with fixation of susceptibility to races 1 and D and resistance to races A, B and C. It is concluded that the Pl6 gene is not a “strong” gene, giving resistance to all downy mildew races, but rather a cluster of genes, each providing resistance to one, or a few, downy mildew races. The genes giving resistance to races 1 and D, on one hand, and to races A, B and C, on the other hand, must be very closely linked, with about 0.6 cM between the two groups.
    Type of Medium: Electronic Resource
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