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  • Articles: DFG German National Licenses  (3)
  • 1995-1999  (3)
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  • Articles: DFG German National Licenses  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Development of a polysacharide degrading strain of Saccharomyces cerevisiae (1998)
    Springer
    Biotechnology techniques 12 (1998), S. 615-619 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries. © Rapid Science Ltd. 1998
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008829129516
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic and fermentation properties of the K2 killer yeast, Saccharomyces cerevisiae T206 (1998)
    Springer
    Antonie van Leeuwenhoek 73 (1998), S. 263-269 
    Details
    ISSN: 1572-9699
    Keywords: Saccharomyces cerevisiae ; karyotyping ; killer yeast ; fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saccharomyces cerevisiae T206 K+R+, a K2 killer yeast, was differentiated from other NCYC killer strains of S. cerevisiae on the basis of CHEF-karyotyping and mycoviral RNA separations. Genomic DNA of strain T206 was resolved into 13 chromosome bands, ranging from approximately 0.2 to 2.2 Mb. The resident virus in strain T206 yielded L and M RNA species of approximately 5.1 kb and 2.0 kb, respectively. In micro-scale vinifications, strain T206 showed a lethal effect on a K-R- mesophilic wine yeast. Metabolite accumulation and toxin activity were measured over a narrow pH range of 3.2 to 3.5. Contrary to known fermentation trends, the challenged fermentations were neither stuck nor protracted although over 70% of the cell population was killed. Toxin-sensitive cells showed cytosolic efflux.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1001190125846
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning of two b-xylanase-encoding genes from Aspergillus niger and their expression in Saccharomyces cerevisiae (1997)
    Springer
    Biotechnology letters 19 (1997), S. 411-415 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two endo-b-1,4-xylanase-encoding genes were amplified from Aspergillus niger ATCC 90196 mRNA, inserted between the yeast ADH2 promoter and terminator sequences (genes designated XYN4 and XYN5) and expressed in Saccharomyces cerevisiae. The nucleotide sequences of the XYN4 and XYN5 genes revealed that both genes encode 211-amino acid proteins that are 92% identical to each other. Both the Xyn4 and Xyn5 enzymes have pH and temperature optima of pH 4 and 60°C, respectively. Autoselective S. cerevisiae strains were developed that allowed b-xylanase production and secretion in complex medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018327623422
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    Paper (German National Licenses)
    Fulltext
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