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  • Articles: DFG German National Licenses  (2)
  • 1995-1999  (2)
  • 1
    Electronic Resource
    Electronic Resource
    High molecular mass glycoproteins associated with the siliceous scales and bristles of Mallomonas splendens (Synurophyceae) may be involved in cell surface development and maintenance (1996)
    Springer
    Planta 199 (1996), S. 219-228 
    Details
    ISSN: 1432-2048
    Keywords: Algae (extracellular matrix) ; Cell surface ; Extracellular matrix (immunocytochemistry) ; Glycoprotein ; Mallomonas (scale case) ; Synurophyceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elaborate scale case of Mallomonas splendens (Synurophyceae) consists of an overlapping arrangement of siliceous scales. In addition, siliceous bristles are attached to specialized base plate scales located at both the anterior and posterior ends of the cells. We have generated monoclonal antibodies against molecules associated with the scale case of M. splendens. One of these antibodies, designated MsS.H9, labelled a proteinaceous epitope of high-molecular-mass cell surface glycoproteins. Immunofluorescence and immunoelectron microscopy demonstrated that only two regions of M. splendens scale cases were labelled by MsS.H9, namely, the upper surface of the scales that contact neighboring scales and the bases of the bristles. Immunoelectron microscopy using thin sections of M. splendens cells showed these labelling sites corresponded to the amorphous material at the sites of scale-to-scale overlap and to a fibrillar complex located at scale-to-bristle attachment sites. Scales and bristles of M. splendens are formed within the cell, in silica deposition vesicles. Immunolabelling of cell sections containing developing scales and bristles showed that MsS.H9 labelling sites were present very early in the formation of these cell surface components. MsS.H9 labelling was also found associated with developing flagellar hairs whereas no labelling was detected on these structures after their deployment onto the flagellum. The location of MsS.H9 labelling sites strongly suggests that the molecule(s) recognized by the antibody plays a role in the adhesion of the individual components making up the scale case of M. splendens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196562
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Substratum adhesion and gliding in a diatom are mediated by extracellular proteoglycans (1997)
    Springer
    Planta 203 (1997), S. 213-221 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Adhesion ; Diatom ; Extracellular matrix ; Gliding (diatom) ; Proteoglycan ; Stauroneis (extra cellular proteoglycans)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Diatoms are unicellular microalgae encased in a siliceous cell wall, or frustule. Pennate diatoms, which possess bilateral symmetry, attach to the substratum at a slit in the frustule called the raphe. These diatoms not only adhere, but glide across surfaces whilst maintaining their attachment, secreting a sticky mucilage that forms a trail behind the gliding cells. We have raised monoclonal antibodies to the major cell surface proteoglycans of the marine raphid diatom Stauroneis decipiens Hustedt. The antibody StF.H4 binds to the cell surface, in the raphe and to adhesive trails and inhibits the ability of living diatoms to adhere to the substratum and to glide. Moreover, StF.H4 binds to a periodate-insensitive epitope on four frustule-associated proteoglycans (relative molecular masses 87, 112, and 〉200 kDa). Another monoclonal antibody, StF.D5, binds to a carbohydrate epitope on the same set of proteoglycans, although the antibody binds only to the outer surface of the frustule and does not inhibit cell motility and adhesion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050184
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    Paper (German National Licenses)
    Fulltext
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