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  • Articles: DFG German National Licenses  (6)
  • 1990-1994  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Identification of a RAPD marker linked to the oat stem rust gene Pg3 (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 702-705 
    Details
    ISSN: 1432-2242
    Keywords: Polymerase chain reaction ; Disease resistance loci ; Oat DNA ; RAPD primers ; Rapid DNA extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The feasibility of identifying molecular markers linked to disease resistance genes in oats was investigated utilizing random primers in conjunction with polymerase chain reaction technology. A pair of near-isogenic oat lines were screened for polymorphic DNA fragments linked to the stem rust resistance gene Pg3. Two primers were identified which amplified DNA fragments that were polymorphic between the lines analyzed. One primer (ACOpR-2) was shown to be completely linked to the Pg3 locus; the other primer was not linked to either the ACOpR-2 or the Pg3 loci. This type of analysis, combined with rapid leaf disc DNA extraction techniques, offers an effective means of identifying useful molecular markers and of applying them to plant breeding selection strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225008
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cytological and molecular characterization of a chromosome interchange and addition lines in Cadet involving chromosome 5B of wheat and 6Ag of Lophopyrum ponticum (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 827-832 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Lophopyrum ; Translocation ; Repetitive DNA sequence ; C-banding ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Efforts to transfer wheat curl mite (Eriophyes tulipae Keifer) resistance from Lophopyrum ponticum 10X (Podb.) Love to bread wheat (Triticum aestivum L.) have resulted in the production of a number of cytogenetic stocks, including an addition line of 6Ag, a “ditelo” addition line, and a wheat-Lophopyrum translocation line. Characterization of these lines with C-banding, in situ hybridization with a Lophopyrum species-specific repetitive DNA probe (pLeUCD2), and Southern blotting with pLeUCD2 and a 5S ribosomal DNA probe (pScT7) confirmed that the distal portion of the short arm of 6Ag was translocated onto the distal portion of 5BS (5BL. 5BS-6AgS). It was also determined that the “ditelo” addition was an acrocentric chromosome of 6AgS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212608
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of Thinopyrum distichum NORs in wheat×Thinopyrum amphiploids and their backcross generations (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 363-368 
    Details
    ISSN: 1432-2242
    Keywords: Amphiplasty ; Wheat ; Thinopyrum ; Nucleoli ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The C-banding pattern of the satellited chromosomes in Thinopyrum distichum and Triticum durum was established. Both T. durum and Th. distichum contained two pairs of satellited chromosomes, which could be distinguished from one another. In the amphiploids [T. durum×Th. distichum (2x=56)] and in the backcross T. durum/(T. durum×Th. distichum)2, BC1F3, and BC1F5 (2n = 42) the satellite was visible on only 1B and 6B of T. durum. The vector pTa 71 containing the rRNA gene from wheat hybridized to two pairs of chromosomes (four hybridization sites) in T. durum and Th. distichum, to eight sites in the amphiploid hybrid (2n=56), and to six sites in the backcross populations BC1F1. BC1F3, and BC1F5 (2n=42). The two satellite pairs in Th. distichum could be distinguished by the chromosomal location of the rRNA site (median or subterminal) and by the centromere position. One copy of each pair was present in the BC1F1, but in the BC1F3 and BC1F5 populations the pair with the subterminal location of rRNA genes was absent. Silver nitrate staining indicated that the rRNA genes of T. durum did not completely suppress those of Th. distichum. The octoploid amphiploid (2n = 56) contained a maximum of four large and four small nucleoli and the hexaploid BC1s (2n=42), four large and two small nucleoli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228677
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A repeated sequence probe for the C genome in Avena (Oats) (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 1-7 
    Details
    ISSN: 1432-2242
    Keywords: Repeated DNA ; Ribosomal DNA ; Avena ; C genome ; Speciation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genus Avena consists of at least 23 species composed of three ploidy levels. Cytogenetic analysis has characterised four distinct karyotypes. These are the A, B, C and D genomes. We have isolated a repeated sequence clone that can be used for the detection of the C genome in Avena by filter hybridization techniques. This clone, termed RS-1, is a genomic DNA clone containing at least one highly repeated sequence that is abundant in Avena species containing the C genome. This sequence or a related sequence is also present, but at much reduced levels, in species that do not contain the C genome. Because of its abundance and the characteristic Southern blot pattern, we have termed this clone a C genome specific clone. We have also done similar analysis of the Avena genus using a rDNA clone from wheat. The results of these experiments demonstrate that clearly definable C genome-specific markers can be identified with both probes. These molecular probes can be useful in studying the genomic relationships of Avena and can provide some clues as to the origin of the cultivated Avena species. These results can, therefore, provide breeders with directions for the efficient transfer of desirable traits of wild Avena species into commencal varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223778
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A novel intergeneric hybrid in the Triticeae: Triticum aestivum x Psathyrost achys juncea (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 45-48 
    Details
    ISSN: 1432-2242
    Keywords: Russian wild rye ; Wheat ; Chromosome pairing ; Meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two hybrid embryos of intergeneric origin between Triticum aestivum cv Fukuho (2n=6x=42, AABBDD) and Psathyrostachys juncea (2n=2x=14, NN) were successfully rescued. One hybrid plant had the expected chromosome number of 28 (ABDN), whereas the second plant had 35 chromosomes. The average meiotic chromosome pairing in the 35-chromosome hybrid was 21.87 univalents + 6.38 bivalents + 0.11 trivalents + 0.009 quadrivalents, which indicates that two copies of the N genome were present. Chromosome pairing in the 28-chromosome hybrid was low (1.35 bivalents), and pointed out the lack of homology between the wheat genomes and the P. juncea genome. These new hybrids showed some necrosis and chlorosis, which caused severe floral abortion in the plant that had 35 chromosomes. These problems became gradually less severe after 18 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223785
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Cytogenetic analysis of three Hordeum x Elymus hybrids (1993)
    Springer
    Euphytica 72 (1993), S. 115-119 
    Details
    ISSN: 1573-5060
    Keywords: Critesion ; Hordeum ; Elymus ; genome formula ; intergeneric hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Meiotic chromosome pairing in three Hordeum x Elymus hybrids was analyzed. Hybrid plants of H. jubatum (HHHH) x E. canadensis (SSHH) showed a low level of chromosome pairing, but the observation of trivalents indicated the presence of three H genomes as expected. In the hybrid plants of H. parodii (HHHHHH) x E. canadensis (SSHH), the level of chromosome pairing was rather low, but up to three trivalents per pollen mother cell could be detected rather infrequently. In the progeny of H. parodii (HHHHHH) x E. scabriglumis (SSHH??), chromosome pairing was quite frequent with the occasional occurrence of quadrivalents and pentavalents. This can be explained if the genomic constitution of E. scabriglumis is assumed to be SSHHHH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023779
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    Paper (German National Licenses)
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