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  • Articles: DFG German National Licenses  (5)
  • 1985-1989  (5)
  • Lipid A  (2)
  • dog  (2)
  • 16.16d  (1)
  • 43.35d  (1)
Source
  • Articles: DFG German National Licenses  (5)
Material
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Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Scanning near-field acoustic microscopy (1989)
    Springer
    Applied physics 48 (1989), S. 89-92 
    Details
    ISSN: 1432-0649
    Keywords: 16.16d ; 43.35d
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Scanning near-field acoustic microscopy (SNAM) is a new method for imaging the topography of nonconducting surfaces at a potential lateral resolution in the sub-micron range. The basic element of this method is a distance sensor consisting of a sharply pointed vibrating tip, which is part of a high-Q quartz resonator driven at its resonance frequency. The decrease of the resonance frequency or of the amplitude of vibration when an object comes into the proximity of the tip serves as the important signal. The dependence of this signal on pressure and composition of the coupling gas shows that the hydrodynamic forces in the gas are responsible for the coupling between object and tip. The sensor is incorporated into a scanning device. Well-resolved line scans of a grating of 8 μm periodicity, a lateral resolution of 3 μm and a vertical resolution of 5nm have been achieved in our first experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694423
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The effect of prehepatic insulin administration on alanine flux rates in diabetic dogs (1987)
    Springer
    Diabetologia 30 (1987), S. 402-408 
    Details
    ISSN: 1432-0428
    Keywords: Alanine metabolism ; insulin-dependent diabetes ; dog ; isotopic study ; portal insulin infusion ; artificial B cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The in vivo flux rates of glucose (6-3H-glucose) and of alanine (U-14C-alanine) were measured in insulin-dependent chronically diabetic dogs which were infused with insulin employing a bedside-type artificial B cell and either the peripheral or the portal venous route. In comparison with non-diabetic control animals the diabetic dogs had near-normal patterns of glucose metabolism and pancreatic glucagon regardless of the route of insulin administration. They also showed reduced basal portal but moderately elevated peripheral insulin levels on peripheral and near-normal peripheral values on portal insulin infusion. Both concentration and production rates of alanine were reduced on peripheral (0.142±0.016mmol/l, 4.73±0.49 μmol·kg−1·min−1, p〈 0.05) but normal on portal insulin (0.206±0.030 mmol/l, 6.33±0.63 μmol·kg−1 ·min−1). The alanine clearance was slightly elevated or normal in the diabetic dogs, and the glucose production from alanine showed a strongly delayed response to an exogenous glucose load on either route of insulin administration. It is concluded that the peripheral hyperinsulinism during posthepatic insulin administration stimulates glucose utilisation to a normal extent, but inhibits the provision of amino groups in resting muscle. Alanine synthesis is thereby reduced, and the carbon moieties are shunted from glucose into circulating lactate. Long-term studies are needed to elucidate the role of the liver under these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292542
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Alterations in alanine metabolism in diabetic dogs during short-term treatment with an artificial B cell (1985)
    Springer
    Diabetologia 28 (1985), S. 763-768 
    Details
    ISSN: 1432-0428
    Keywords: Artificial B cell ; dog ; experimental diabetes ; alanine metabolism ; gluconeogenesis ; tracer study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The flux rates of plasma glucose and alanine were studied isotopically (6-3H-glucose and U-14C-alanine simultaneously) in resting chronically diabetic dogs during short-term treatment with an artificial B cell where the insulin was infused into a peripheral vein. Despite perfect blood glucose control and normal glucose flux rates, the concentration and rates of appearance and disappearance of alanine were significantly elevated in the diabetic animals before, during and after an exogenous glucose load. The incorporation of the carbon moiety of alanine into circulating glucose was also increased, but diminished to a near-normal extent when exogenous glucose was given. The plasma clearance rates for alanine in the diabetic dogs were normal throughout the study. It is concluded that normal blood glucose control in diabetes does not necessarily mean normalization of the entire metabolic network. On the basis of peripheral hyperinsulinaemia alanine formation from glucose and branched chain amino acids is elevated in muscle. This may explain increased flux of alanine despite normal blood glucose control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265025
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The lipopolysaccharide of the green sulfur bacterium Chlorobium vibrioforme f. thiosulfatophilum (1987)
    Springer
    Archives of microbiology 149 (1987), S. 125-129 
    Details
    ISSN: 1432-072X
    Keywords: Chlorobiaceae ; Chlorobium vibrioforme ; Degraded polysaccharide ; DOC-PAGE ; Lipid A ; Lipopolysaccharide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cell wall lipopolysaccharide of the green sulfur bacterium Chlorobium vibrioforme f. thiosulfatophilum was obtained by the phenol-chloroform-petroleum ether and the hot phenol-water methods, respectively. It contained mannose, glucose, galacturonic acid, glucosamine, glycine, and small amounts of rhamnose, galactose and glucuronic acid. In addition to d-glycero-d-mannoheptose, the corespecific constituents 2-keto-3-deoxyoctonate and l-glycero-d-mannoheptose were found. Polyacrylamide gel-electrophoresis in the presence of sodium deoxycholate gave no indication for the presence of O-specific repeating units. Degradation of the lipopolysaccharide required 10% acetic acid (100° C, 2 h). The lipid A moiety contained the total of glucosamine of the lipopolysaccharide as well as small amounts of 2,3-diamino-2,3-dideoxy-glucose. It was phosphate-free. The fatty acid spectrum comprised 3-OH-14:0, 3-OH-16:0, and iso-3-OH-18:0 besides little 12:0, 14:0 and 16:0. Hydroxylaminolysis and sodium methylate treatment revealed all of the three hydroxy fatty acids to be amidebound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425077
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The lipopolysaccharide of the phototrophic bacterium Ectothiorhodospira vacuolata (1988)
    Springer
    Archives of microbiology 149 (1988), S. 245-248 
    Details
    ISSN: 1432-072X
    Keywords: Ectothiorhodospira vacuotata ; Lipopolysaccharide ; Lipid A ; DOC-PAGE ; Phototrophic bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipopolysaccharide of Ectothiorhodospira vacuolata was obtained by the phenol-water procedure. It contained a 3-O-methyl-hexose, glucose, galacturonic and glucuronic acids. The finding of d-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate (tentatively identified) suggested a core-structure. The lipid fraction of the lipopolysaccharide contained phosphate and both, 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine. The major fatty acids were amine-bound 3-OH-10:0 and 3-OH-12:0 and esterbound 14:0 and 16:0 Sodium deoxycholate gel-electrophoresis, showing a single band only, indicated R-type character of the lipopolysaccharide of Ectothiorhodospira vacuolata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422012
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    Paper (German National Licenses)
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