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  • Articles: DFG German National Licenses  (2)
  • 1985-1989  (2)
  • Agaricus  (2)
  • Computational Chemistry and Molecular Modeling
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  • Articles: DFG German National Licenses  (2)
Material
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Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Physical mapping of the mitochondrial genome of the cultivated mushroom Agaricus brunnescens (= A. bisporus) (1988)
    Springer
    Current genetics 14 (1988), S. 43-49 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; MtDNA ; Restriction map ; Inverted repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNA from the commercial mushroom Agaricus brunnescens Peck [= A. bisporus (Lange) Imbach] was purified by cesium chloride/bisbenzimide gradient centrifugation. A physical map of the mtDNA fragments produced by BamHI, EcoRl, and PvuII digestion was generated by filter hybridizations with radiolabelled BamHI mtDNA probes. The A. brunnescens mtDNA was a circular molecule 136 kilo-basepairs (kbp) in length and contained an inverted repeat between 4.6 and 9.2 kbp in size. Orientational isomers of the mitochondrial genome were not detected. The positions of six genes were located on the A. brunnescens mtDNA map by heterologous hybridization. No coding function has yet been ascribed to the inverted repeat. The large rRNA gene was located on the smaller single copy region. The genes for cytochrome b, cytochrome oxidase (subunit III), ATPase (subunits 8 and 6) and the small rRNA were located on different regions of the larger single copy region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405852
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The mitochondrial DNAs of Agaricus: heterogeneity in A. bitorquis and homogeneity in A. brunnescens (1985)
    Springer
    Current genetics 9 (1985), S. 127-132 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; Mitochondrial DNA ; Restriction pattern polymorphism ; Restriction endonuclease analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNAs were isolated from four cultivated strains of the commercial two-spored mushroom Agaricus brunnescens (bisporus) and from ten isolates of the four spored mushroom Agaricus bitorquis. Digestion of the fungal mitochondrial DNA with restriction endonucleases yielded numerous fragments. Summation of the fragment sizes gave a mitochondrial DNA size of 98.3 ± 2.4 kilobases (kb) (64.9 x 106 daltons) for A. brunnescens. The size of the mitochondrial DNA ranged from 148.5 ± 10.8 kb (98.0 x 106 daltons) to 176.3 ± 12.0 kb (116.4 x 106 daltons) for A. bitorquis. The restriction patterns, produced by a variety of endonucleases, were identical for all four isolates of A. brunnescens. The ten isolates of A. bitorquis demonstrated extensive restriction pattern heterogeneity and have been tentatively assigned into four groups. Approximately 60% of the A. bitorquis mitochondrial DNA restriction fragments show sequence homology with A. brunnescens mitochondrial DNA based on DNA — DNA hybridizations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436960
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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