ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Conditions in continuous culture were identified under which thermostable α-amylase and pullulanase were overproduced and excreted by Clostridium thermohydrosulfuricum DSM 567. Maximal amounts of these enzymes were formed at a dilution rate of 0.05/h at pH of 6.5 and under starch limitation. Unlike the results obtained in batch culture, more than 90% of the enzymes were released into the culture fluid. In addition, the concentration of these enzymes was raised 50- to 100-fold. A shift in fermentation products (more lactate and less acetate production) was observed, when the concentration of ferrous ions in the medium was decreased from 23 μM to 6.5 μM.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00257257
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