ISSN:
1365-2109
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
In order to confirm the suitability of cholestane (CLS) for determination of lipid digestibilities in prawn, Marsupenaeus japonicus, the movements of dietary triacylglycerol and CLS in its digestive tract were examined. In this present study, the double labelling method was evaluated using [3H]-triolein and [14C]-CLS, which was chemically synthesized from [14C]-cholesterol. Three test diets containing different levels of lipid (0, 80 and 100 g kg−1 dry diet) with [3H]-triolein (150 MBq kg−1 diet) and [14C]-CLS (150 MBq kg−1 diet) were prepared. One prawn was stocked into a 1 L Erlenmeyer flask containing seawater at 25 °C. After the prawns had been fed on 1% of body weight of the labelled diets, they were transferred to another Erlenmeyer flask and held for 1, 3, 6, 12, 24, 36 and 96 h after feeding. At every collection time, radioactivity of the hemolymph, stomach, midgut gland, midgut, hindgut, muscle and faeces was determined by liquid scintillation counter. All analyses were performed in triplicate with one prawn per collection time per test diet.In the prawns administrated labelled diets, over 75% of the total radioactivity was recovered in faeces at 96 h after feeding. The ratio of [3H] to [14C] in the gastrointestinal tract of the prawn did not fluctuate for 96 h after feeding. Thus, triolein and CLS were shown to move at the same speed in the gastrointestinal tract. The results demonstrate that CLS satisfied one requisite for markers in the digestibility measurement of dietary lipids in the prawn.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1355-557x.2001.00055.x
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