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  • Articles: DFG German National Licenses  (2)
  • Agrobacterium  (1)
  • Ankle joint  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 347 (1978), S. 651-651 
    ISSN: 1435-2451
    Keywords: Ankle joint ; corrective procedures ; Sprunggelenksfrakturen ; Fehlstellungen ; Korrekturmöglichkeiten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Im Rahmen einer AO-Sammelarbeit wurden aus 6 Kliniken Deutschlands insgesamt 135 Korrektureingriffe am oberen Sprunggelenk der Jahre 1962–1974 ausgewertet. Es wird über Indikationsstellung, Komplikationen und Behandlungsergebnisse berichtet. Die besten Ergebnisse wurden each Korrektureingriffen bei ursprünglichen Luxationsfrakturen vom Typ A und B erreicht. Hier beschränkten sich die Eingriffe vorwiegend auf die Stabilisierung einer Außen- oder Innenknöchelpseudarthrose. Bei Luxationsfrakturen vom Typ C überwiegen die Verängerungsosteotomien der Fibula — hier konnten in 43 % der Fälle gute Spätergebnisse erreicht wurden.
    Notes: Summary A total of 135 corrective procedures on the ankle joint performed in 6 German hospitals from 1962 to 1974 is reviewed. Indications, results of treatment, and complications are reported. Best results were achieved with corrective procedures following dislocation fractures of Type A or B. In most cases of this category, non-union of the lateral or medial ankle required stabilization. For old dislocation fractures of Type C, elongation osteotomy of the fibula was performed most frequently. Good longterm results were obtained in 43 % of cases.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Key words Peppermint ; Transformation ; Agrobacterium ; GUS ; Transgenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first transgenic peppermint (Mentha×piperita L. cultivar Black Mitcham) plants have been obtained by Agrobacterium-mediated transformation by cocultivation with morphogenically responsive leaf explants. Basal leaf explants with petioles, from leaves closest to the apex of in-vitro-culture-maintained shoots (5 cm), exhibited optimal shoot organogenetic responsiveness on medium supplemented with thidiazuron (8.4 µm). Shoot formation occurred at sites of excision on the leaf blade and petiole either directly from cells of the explant or via a primary callus. Analyses of transient GUS activity data indicated that DNA delivery by microprojectile bombardment was more effective than Agrobacterium infection. However, no transgenic plants were obtained from over 22,000 leaf explants after particle bombardment. Cocultivation of leaf explants with Agrobacterium strain EHA 105 and kanamycin selection produced transgenic plants. Greater transient and stable -glucuronidase (GUS) activities were detected in explants or propagules transformed with the construct where gusA was driven by the pBISN1 promoter rather than a CaMV 35S promoter. Eight plants were subsequently regenerated and verified as transgenic based on detection of the nptII transgene by PCR and Southern blot analyses. The Southern analyses indicated that the plants were derived from eight unique transformation events. All transgenic plants appeared morphologically normal. Analyses of GUS activities in leaves sampled from different portions of these transgenic plants, 10 months after transfer to the greenhouse, indicated that six out of the eight original regenerants were uniformly transformed, i.e., did not exhibit chimeric sectors.
    Type of Medium: Electronic Resource
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