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  • Articles: DFG German National Licenses  (3)
  • Anabaena  (2)
  • gibberellin  (1)
  • 1
    ISSN: 1432-2048
    Keywords: Anabaena ; Heterocyst ; Iron-superoxide dismutase (immunogold labeling)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iron superoxide dismutase (Fe-SOD; EC 1.15.1.1) was isolated from the nitrogen-fixing cyanobacterium Anabaena cylindrica Lemm. Polyacrylamide gel electrophoresis separated the purified protein into three closely running, enzymatically active bands. The molecular weight of the enzyme was estimated by gel filtration to be about 40 kDa. Polyclonal antibodies were produced by immunization of rabbits with the isolated enzyme, and were purified on a column of protein A-Sepharose. The Fe-SOD antibody reacted with the purified Fe-SOD and also specifically recognized the protein in extracts of A. cylindrica. In the extracts, anti-Fe-SOD did not cross-react with Mn-SOD, an enzyme which belongs to an SOD class displaying high homology of primary and three-dimensional structure with respect to Fe-SOD. Iron superoxide dismutase was localized in heterocysts by immunogold labeling and transmission electron microscopy. These results are the first in-situ evidence for the presence of SOD in the cells specialized for nitrogenase activity.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 137 (1991), S. 151-155 
    ISSN: 1573-5036
    Keywords: Anabaena ; Arthrobacter ; Azolla ; erythromycin ; novobiocin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The leaf cavities and the sporocarps of the water fernAzolla contain bacteria belonging to the genusArthrobacter Conn and Dimmick. The number of these bacteria can be decreased by treating the plants with novobiocin and erythromycin. The latter antibiotic kills theAnabaena, soAnabaena free plants can be obtained. In these plants the bacteria disappear almost completely. The novobiocin does not affect the heterocyst differentiation, but it decreases the N2-fixation activity by affecting the synthesis of the dinitrogenase reductase.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 11 (1987), S. 75-78 
    ISSN: 1573-5044
    Keywords: Crocus sativus L. fruit development in vitro ; parthenocarpy ; 2,4-dichlorophenoxyacetic acid ; benzyladenine ; gibberellin ; abscisic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro development of parthenocarpic fruits of Crocus sativus L. was induced by culturing ovaries on MS agar medium supplemented with growth-regulators (2,4-D, GA3 and BAP). Amongh these, 2,4-D was the most effective in promoting fructification. The fructigenic activity was independent of both the stage at which the ovaries were excised (before, during or after anthesis) and pollination of the stigmas. Unlike the above compounds, abscisic acid inhibited fructification.
    Type of Medium: Electronic Resource
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