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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 50-52 
    ISSN: 1573-0972
    Keywords: β-Amylase ; Bacillus ; soil isolate ; thermotolerant enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An isolate from a Hong Kong soil sample which produced β-amylase was identified as a thermotolerant strain ofBacillus circulans with a growth range of 35 to 55δC. The β-amylase was stable at 45°C for 30 min but lost half of its activity after 30 min at 50°C. Maximum specific activity of β-amylase (36.2 units/mg protein) in the culture broth was detected after 36 h of cultivation at 45°C in a medium containing soluble starch, beef extract, coconut water and inorganic salts.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 10 (1994), S. 597-598 
    ISSN: 1573-0972
    Keywords: Amylases ; Bacillus ; enzyme ; maltose ; starch ; thermotolerant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A thermotolerant β-amylase was purified from Bacillus circulans S31 isolated from soil in Hong Kong. The purified enzyme has an M r of 64 kDa and was stable at 50°C and pH 7.0 for 30 min. Its K m for starch was 0.9 mg/ml with a V max of 0.3 mg/min. It was not activated by any metal ion although sulphydrys reagents were inhibitory.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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