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  • Articles: DFG German National Licenses  (8)
  • tomato  (5)
  • Barley aleurone  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Localization of ATPase in the endoplasmic reticulum and golgi apparatus of barley aleurone (1987)
    Springer
    Protoplasma 138 (1987), S. 73-88 
    Details
    ISSN: 1615-6102
    Keywords: ATPase ; Barley aleurone ; Endoplasmic reticulum ; Gibberellic acid ; Golgi apparatus ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytochemical localization of adenosine triphosphatase (ATPase) was studied in the aleurone layer of barley (Hordeum vulgare L. cv. Himalaya). Isolated barley aleurone layers secrete numerous enzymes having acid phosphatase activity, including ATPase. The secretion of these enzymes was stimulated by incubation of the aleurone layer in gibberellic acid (GA3). ATPase was localized using the metal-salt method in tissue incubated in CaCl2 with and without GA3. In sections of tissue incubated without GA3, cytochemical staining was confined to a narrow band of cytoplasm adjacent to the starchy endosperm and to the cell wall of the innermost tier of aleurone cells. Cytochemical staining was absent from the organelles of tissues not treated with GA3. In tissue incubated in the presence of GA3, cytochemical staining was evident throughout the cytoplasm and cell walls of the tissue. In the cell wall, electron-dense deposits were found only in digested channels. The cell-wall matrix of GA3-treated aleurone did not stain, indicating that it does not permit diffusion of enzyme. In the cytoplasm of GA3-treated aleurone, all organelles except microbodies, plastids, and spherosomes stained for ATPase activity; endoplasmic reticulum (ER), Golgi apparatus, and mitochondria showed intense deposits of stain. The ER of the aleurone is a complex system made up of flattened sheets of membrane, which may be associated with both the Golgi apparatus and the plasma membrane. The dictyosome did not stain uniformly for ATPase activity; rather there was a gradation in staining of the cisternae from thecis (lightly stained) to thetrans (heavily stained) face. Vesicles associated with dictyosome cisternae also stained intensely as did the protein bodies of GA3-treated aleurone cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281016
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of α-amylase isozymes within the endomembrane system of barley aleurone (1990)
    Springer
    Protoplasma 154 (1990), S. 16-24 
    Details
    ISSN: 1615-6102
    Keywords: α-Amylase isozymes ; Barley aleurone ; Endoplasmic reticulum ; Golgi apparatus ; Immunocytochemistry ; Intracellular transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The localization of α-amylase (EC 3.2.1.1) in barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts was studied using electron microscope immunocytochemistry. Antibodies were raised against total barley α-amylase, i.e., α-amylase containing both highisoelectric point (high-pI) and low-pI isoforms, as well as against purified high- and low-pI isoforms. All antibodies localized α-amylase to the endoplasmic reticulum (ER) and Golgi apparatus (GApp) of the aleurone cell, and various controls showed that the labeling was specific for α-amylase. Labeling of protein bodies and spherosomes, which are the most abundant organelles in this cell, was very low. There was no evidence that α-amylase isoforms were differentially distributed within different compartments of the endomembrane system. Rather, both high- and low-pI isoforms showed the same pattern of distribution in ER and in the cis, medial, and transregions of the GApp. We conclude that in the Himalaya cultivar of barley, all isoforms of α-amylase are transported to the plasma membrane via the GApp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01349531
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Estimating viability of plant protoplasts using double and single staining (1986)
    Springer
    Protoplasma 135 (1986), S. 80-87 
    Details
    ISSN: 1615-6102
    Keywords: Barley aleurone ; Fluorescein diacetate ; Propidium iodide ; Protoplasts ; Viability determination ; Vital stains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The utility of numerous dyes for determining the viability of barley (Hordeum vulgare L. cv. Himalaya) aleurone protoplasts was studied. Protoplasts isolated from the barley aleurone layer synthesize and secrete α-amylase isozymes in response to treatment with gibberellic acid (GA) and Ca2+. These cells also undergo dramatic morphological changes which eventually result in cell death. To monitor the viability of protoplasts during incubation in GA and Ca2+, several types of fluorescent and nonfluorescent dyes were tested. Evans blue and methylene blue were selected as nonfluorescent dyes. Living cells exclude Evans blue, but dead cells and cell debris stain blue. Both living and dead cells take up methylene blue, but living cells reduce the dye to its colorless form whereas dead cells and cell debris stain blue. The relatively low extinction coefficient of these dyes sometimes makes it difficult to distinguish blue-stained cells against a background of blue dye. Several types of fluorescent dyes were tested for their ability to differentially stain dead or living cells. Tinopal CBS-X, for example, stains only dead cells, and its high extinction coefficient allows its ultraviolet fluorescence to be recorded even when preparations are simultaneously illuminated with visible light. To double-stain protoplasts, the most effective stain was a combination of fluorescein diacetate (FDA) and propidium iodide (PI). By employing a double-exposure method to record the fluorescence from cells stained with both FDA and PI, dead and living cells could be distinguished on the basis of fluorochromasia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01277001
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    High salt tolerance potential in Lycopersicon species during germination (1986)
    Springer
    Euphytica 35 (1986), S. 575-582 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; salinity ; germination ; germplasm ; breeding ; salt-tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The potential to improve seed germination responses to salinity was evaluated for 13 accessions representing six wild Lycopersicon species and 20 accessions of L. esculentum. Germination response times increased in all accessions at 100 mM NaCl. Analysis indicated that one accession of L. peruvianum (PI126435) germinated faster under high salinity than all other accessions and was closely followed by L. pennellii (LA716). The fastest germinating L. esculentum accession, PI174263, ranked third. Additional wild ecotypes exhibiting rapid germination at 100 mM NaCl were identified among L. pimpinellifolium and L. peruvianum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021866
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    A greenhouse screening method forPyrenochaeta lycopersici resistance in tomatoes (1989)
    Springer
    Euphytica 40 (1989), S. 187-191 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; Pyrenochaeta lycopersici ; corky root ; brown root rot ; resistance ; greenhouse screening method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse screening method for corky root (Pyrenochaeta lycopersici) resistance in the tomato (Lycopersicon esculentum) is described in detail. In determining the reliability of the method, known resistance sources within wild asccessions, commerical cultivars and breeding lines were evaluated for resistance in fields naturally infested with corky root and their response compared under greenhouse conditions in soil heavily infested with corky root and artifically maintained at temperatures between 10–13°C. The procedure developed can be used year round and improves the efficiency of recovering resistant progeny in large segregrating populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024510
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Generation means analysis of right-censored response-time traits: Low temperature seed germination in tomato (1990)
    Springer
    Euphytica 48 (1990), S. 239-244 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; cold tolerance ; seed germination ; genetic analysis ; missing data ; response-time data
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In studies to determine the inheritance of response-time traits, such as time to seed germination, some viable individuals may fail to respond during an experiment. If these right-censored observations are ignored, sample means and variances will be underestimated. This is illustrated using data from time to seed germination at 9°C for Lycopersicon esculentum (Mill.) fast germinating PI 120256, slow-germinating T3 and their reciprocal F1, F2 and backcross progeny. This paper presents methods to detect and to accommodate right-censored data in generation means analysis. Genetic interpretations derived from corrected and uncorrected estimates of generation means and variances are compared. Correction for right-censoring increased estimates of environmental and phenotypic variances, and decreased heritability estimates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023656
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Improvement of tomato flavor by genetically increasing sugar and acid contents (1983)
    Springer
    Euphytica 32 (1983), S. 845-855 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; flavor ; sensory evaluation ; soluble solids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Advanced high sugar and acid breeding lines of tomatoes (Lycopersion esculentum Mill.) were rated higher in sweetness, sourness and overall flavor intensity than the standard cultivars Cal Ace or T3. Titratable acidity and soluble solids content were major contributors to differences in overall flavor intensity. The results demonstrate that significant improvement in tomato flavor can be attained by increasing sugar and acid contents in tomato fruits by genetic manipulation. Current evidence indicates that breeding for high soluble solids in horticulturally acceptable tomato cultivars is justified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042166
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Low temperature seed germination of Lycopersicon species evaluated by survival analysis (1982)
    Springer
    Euphytica 31 (1982), S. 869-883 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; low temperature germination ; survival analysis ; high altitude ecotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Low temperature germination responses were evaluated for 18 high altitude accessions representing five wild Lycopersicon species and 19 accessions of L. esculentum which have reputed ability to germinate in the cold. Survival analysis indicated that one accession of L. chilense germinates better at 10°C than PI 120256, the fastest-germinating L. esculentum genotype, and that PI 120256 germinates as well as PI 126435 (L. peruvianum). Additional wild ecotypes exhibiting rapid germination at 10°C were identified from L. peruvianum and L. hirsutum. These ecotypes may possess genetic potential for introgressing cold germination ability into L. esculentum cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039227
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    Paper (German National Licenses)
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