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Transgressive segregation and reciprocal effect for free folic acid content in a red beet (beta vulgaris L.) population (1997)

Wang, Min ; Goldman, Irwin L.

Springer

Euphytica 96 (1997), S. 317-321

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ISSN: 1573-5060

Keywords: free folic acid content ; Beta vulgaris ; transgressive segregation ; reciprocal effect ; broad sense heritability

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract Folic acid is one of the ten essential vitamins in the human diet. Red beet (Beta vulgaris L.) is an excellent source of free folic acid among vegetable plants. Thirty-seven F3 families derived from the cross W364 × Nochowski, sixteen F3 families derived from the reciprocal cross, and the two parents were evaluated for free folic acid content (FFAC) in two locations in Wisconsin in 1994. Parental means of FFAC were 3.4 and 8.8 µg g-1 dry wt. for Nochowski and W364, respectively. The range of FFAC in F3 families was from 2.3 to 15.8 µg g-1 dry wt. The genetic variance among F3 families was large. Transgressive segregation for FFAC was observed in the F3 family distribution, suggesting multiple genes control this trait. FFAC means differed at the two locations. Overall means of the reciprocal crosses were significantly different in FFAC at both locations (P 〈 0.05), suggesting reciprocal effects on FFAC in this population. The broad sense heritability for FFAC in this population was estimated as 0.84.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1003063008648

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Pulsed field separation of large supercoiled and open-circular DNAs and its application to bacterial artificial chromosome cloning (1995)

Wang, Min ; Lai, Eric

Weinheim : Wiley-Blackwell

Electrophoresis 16 (1995), S. 1-7

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ISSN: 0173-0835

Keywords: Cloning ; Bacterial artificial chromosome ; Supercoiled DNA ; Open-circular DNA ; Pulsed field gel electrophoresis ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: We have studied the separation of large (80-300 kbp) supercoiled (SC) DNA in conventional agarose gel electrophoresis, field inversion gel electrophoresis (FIGE) and pulsed field gel electrophoresis (PFGE). DNA migration was measured under a variety of electrophoretic conditions including different switch times, temperatures, agarose concentrations, and voltage gradients. The migration of SC DNA was found to be inversely proportional to its molecular weight in the three electrophoresis systems tested. In conventional agarose electrophoresis, voltage gradient was found to be the determining parameter in the separation of SC DNA. Unlike large linear DNAs, the migration of SC DNA was found to be independent of switch time in PFGE and FIGE. Broad DNA bands were observed in prolonged FIGE runs. In addition, we have also studied the migration of open-circular (OC) DNA (80 and 100 kbp) in pulsed field gel electrophoresis. Eighty kbp OC DNA can migrate into agarose gels under certain pulsed field conditions whereas 100 kbp OC DNA was trapped at the wells. Based on electrophoretic conditions described in this report, we can determine the size of bacterial artificial chromosome (BAC) clones without restriction enzyme digestion and have enriched the percentage of larger size clones in BAC cloning.

Additional Material: 6 Ill.